Ulysis Alexa Fluor 546 Nucleic Acid Labeling Kit/Alexa Fluor 546核酸标记试剂盒
货号：U21652
规格：1 kit
价格：8083
产品类型：核酸标记
品牌：Thermo Fisher
| ULYSIS™ 核酸标记试剂盒提供一种独特的方法来将荧光染料附加到核酸上。该试剂盒中的标记试剂可与鸟嘌呤的 N7 发生反应形成稳定的配合物，而这一反应非常简单快速—仅需使 DNA 发生热变性（5 分钟），加入标签（反应 15 分钟）然后纯化。ULYSIS™ 核酸标记规格：•染料 (Ex/Em)：Alexa Fluor™ 546 (555/570 nm)• 标记反应只需 15 分钟即可完成• 有几种 Alexa Fluor™ 染料颜色可供选择产生的标记探针用于：•斑点、northern 和 Southern 印迹• RNA 和 DNA原位杂交• 多色荧光原位杂交 (mFISH)• 比较基因组杂交 (CGH)•微阵列分析使用通用联动系统进行可靠的标记我们开发了这一系列 ULYSIS™ 试剂盒以确保快速简单地将我们的 Alexa Fluor™ 染料与核酸聚合物中的嘌呤碱基进行偶联。该方法（通用联动系统 (ULS™)）基于铂染料复合物（由 KREATECH Diagnostics 所拥有）的使用，该复合物可与 DNA、RNA、PNA 和寡核苷酸中的鸟嘌呤和腺嘌呤碱基（在较小程度上）的 N7 位点形成稳定的加合物。从而为核酸标记提供了一种可靠的非酶促方法。标记快速、简便标记反应通常仅需 15 分钟，通过使用简单的离心柱程序即可完成从未反应 ULS™ 复合物中分离标记核酸的过程。长于 ∼1,000 个碱基对的 DNA 在标记前需要进行 10 分钟 DNase 消化，这优化了标记并使探针片段化，以实现高效杂交。 |
| 数据： |
Visualization of chromosome 2 using a paint probe labeled with the ULYSIS Alexa Fluor® 546 Nucleic Acid Labeling Kit.A paint probe for chromosome 2 was labeled with the ULYSIS Alexa Fluor® 546 Nucleic Acid Labeling Kit (Cat. no. U21652) and hybridized to human metaphase chromosomes. Image contributed by Joop Wiegant, Leiden University Medical Center, Leiden, The Netherlands. ULYSIS reagent–labeled RNA hybridized to a microarray.ULYSIS reagent–labeled RNA hybridized to a microarray. Poly(A)+ RNA samples from the spleen of an irradiated or unirradiated mouse were labeled using the Alexa Fluor 594 ULS reagent (U21654) or Alexa Fluor® 546 ULS reagent (U21652), respectively. The labeled samples were mixed and hybridized to a cDNA microarray. The image was contributed by Rahul Mitra (Baylor College of Medicine) and Mini Kapoor, Thomas H. Burrows, and Rachel Grier (MD Anderson Cancer Center). |
| 参考文献： |
| 1.A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcription.2.Evaluation of cadmium-induced transcriptome alterations by three color cDNA labeling microarray analysis on a T-cell line.3.Expression profile analysis of microRNA (miRNA) in mouse central nervous system using a new miRNA detection system that examines hybridization signals at every step of washing. |
技术参数 产品优点 • Dye (Ex/Em): Alexa Fluor® 546 (555/570 nm)
• Labeling reaction is complete in as little as 15 minutes
• Available in several Alexa Fluor® dye colors
产品应用 • Dot, northern, and Southern blots
• RNA and DNA in situ hybridization
• Multicolor fluorescence in situ hybridization (mFISH)
• Comparative genome hybridization (CGH)
• Microarray analysis
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