MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), APC
货号：17-5320-82,17-5320-80
规格：100μg,25μg
价格：2476,1029
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：小鼠
抗体亚型：IgG
克隆号：AF6-120.1
荧光染料：APC
抗体类型: | 单抗 | 同型对照： | Mouse IgG2a kappa Isotype Control, APC, eBioscience™ |
免疫原性： | | 用法： | 0.25 µg/test(Flow） |
Description: This AF6-120.1.2 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4.The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed.Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationMHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| II I-Ab Antibody (17-5320-82) in Flow Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.125 µg of Anti-Mouse MHC Class II I-Ab APC (right). Cells in the lymphocyte gate were used for analysis. |
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参考文献： |
| 1. Frontiers in immunologyFcεRI γ-Chain Negatively Modulates Dectin-1 Responses in Dendritic Cells."17-5320 was used in Flow cytometry/Cell sorting to further explore the role of the tyrosine-based activation motif-containing adaptors DAP12 and FcεRI γ-chain in C-type lectin receptor responses."AuthorsPan YG,Yu YL,Lin CC,Lanier LL,Chu CL2. Proceedings of the National Academy of Sciences of the United States of AmericaReduced production of B-1-specified common lymphoid progenitors results in diminished potential of adult marrow to generate B-1 cells.AuthorsBarber CL,Montecino-Rodriguez E,Dorshkind K |
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