Pierce Fast Blocking Buffer / 快速封闭缓冲液/封闭液
货号：37575,37576
规格：500 mL,100 mL
价格：2822,1616
产品类型：辅助试剂
品牌：Thermo Fisher
| Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results for traditional western blotting protocols.Compare and view all available blocking buffersPierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only five minutes, producing results comparable to traditional blockers. Blocking and probing steps with Fast Blocking Buffer prevent nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). This buffer is compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes. |
| 数据： |
Effective Western blot blocking in 5 minutesSerial dilutions of HeLa lysate (12.5μg and down) were separated by SDS-PAGE, transferred to nitrocellulose or PVDF membrane and then blocked for 5 minutes with Thermo Scientific Pierce Fast Blocking Buffer. The blots were probed with mixtures of mouse and rabbit primary antibodies, then with HRP-conjugated secondary antibodies. Wash steps were performed with the Fast Wash Buffer. Finally, blots were detected on film via Pierce ECL Western Blotting Substrate (Part No. 32106). HH3 = Histone H3; CycB = Cyclophilin B. |
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