Pierce Fast Wash Buffer, 10X 快速洗涤缓冲液,10X
货号:37577
规格:1 L
价格:1401
产品类型:免疫印迹/组化
品牌:Thermo Fisher
Pierce Fast Wash Buffer, 10X, for use with Pierce Fast Blocking Buffer. |
数据: |
Serial dilutions of HeLa lysate (12.5μg and down) were separated by SDS-PAGE, transferred to nitrocellulose or PVDF membrane and then blocked for 5 minutes with Thermo Scientific Pierce Fast Blocking Buffer. The blots were probed with mixtures of mouse and rabbit primary antibodies, then with HRP-conjugated secondary antibodies. Wash steps were performed with the Fast Wash Buffer. Finally, blots were detected on film via Pierce ECL Western Blotting Substrate (Part No. 32106). HH3 = Histone H3; CycB = Cyclophilin B. |
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