Pierce Fast Wash Buffer, 10X 快速洗涤缓冲液,10X

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Pierce Fast Wash Buffer, 10X 快速洗涤缓冲液,10X

货号:37577

规格:1 L

价格:1401

产品类型:免疫印迹/组化

品牌:Thermo Fisher

Pierce Fast Wash Buffer, 10X, for use with Pierce Fast Blocking Buffer.
数据:

Serial dilutions of HeLa lysate (12.5μg and down) were separated by SDS-PAGE, transferred to nitrocellulose or PVDF membrane and then blocked for 5 minutes with Thermo Scientific Pierce Fast Blocking Buffer. The blots were probed with mixtures of mouse and rabbit primary antibodies, then with HRP-conjugated secondary antibodies. Wash steps were performed with the Fast Wash Buffer. Finally, blots were detected on film via Pierce ECL Western Blotting Substrate (Part No. 32106). HH3 = Histone H3; CycB = Cyclophilin B.

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