Pierce ECL Western Blotting Substrate ECL蛋白质印迹底物

2024-10-17

Pierce ECL Western Blotting Substrate ECL蛋白质印迹底物

货号:32106,32109,32209

规格:500 mL,50 mL,250 mL

价格:2981,878,2198

产品类型:免疫印迹/组化

品牌:Thermo Fisher

Thermo Scientific Pierce ECL Western 印迹底物是一种用于增强化学发光 (ECL) 的高性价比入门级辣根过氧化物酶 (HRP) 底物,可直接取代成本更高的产品,无需重新优化条件。Pierce ECL Plus Western Blotting 底物特性包括:-灵敏度:低皮克级-稳定性:在 4°C 下工作溶液稳定性达 1 小时,试剂盒稳定性达 1 年-兼容性:硝酸纤维素膜和 PVDF 膜-信号持续时间:1 至 2 小时-推荐一抗浓度:1:1,000– 1:5,000 稀释度 (0.2–10µ g/mL)-推荐的二抗浓度:1:1,000–1:15,000 稀释 (0.07–1.0 µng/mL)Pierce ECL Western 印迹底物在 HRP 酶活性检测方面能够提供与其他标准 ECL 底物同等的可靠性和性能。它可用于检测皮克级抗原,并支持使用摄影或其他成像方法轻松检测 HRP。由于鲁米诺和过氧化物试剂配方与其他市售底物产品相同,因此用户可以直接切换至 Pierce ECL,无需优化探测条件或孵育方案。Pierce ECL Western 印迹底物与 GE Healthcare 原始 Amersham ECL Western 印迹检测试剂具有相同性能。
特点:
-灵敏度:低皮克级-稳定性:在 4°C 下工作溶液稳定性达 1 小时,试剂盒稳定性达 1 年-兼容性:硝酸纤维素膜和 PVDF 膜-信号持续时间:1 至 2 小时-推荐一抗浓度:1:1,000– 1:5,000 稀释度 (0.2–10µ g/mL)-推荐的二抗浓度:1:1,000–1:15,000 稀释 (0.07–1.0 µng/mL)
数据:

ECL Western Blotting Substrate for detection of proteins on nitrocellulose or PVDFβ-actin and β-galactosidase protein in HeLa cell and E. coli lysates were detected by Western blotting. The membranes were blocked with 5% skim milk and probed with primary antibody at 1µg/mL. The membranes were washed and then incubated with 0.2µg/mL of HRP-conjugated goat anti-mouse IgG (Part No. 31430) and then washed again. Working solutions of the Pierce ECL Substrate and GE Healthcare ECL Reagent were prepared according to the manufacturers’ instructions and added to replicate membranes for 1 minute. The membranes were removed from the substrates and placed in plastic sheet protectors and exposed to Thermo Scientific CL-XPosure Film (Part No. 34090) and developed.

技术参数
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