Pierce Albumin/IgG Removal Kit / 白蛋白/ IgG去除试剂盒
货号：89875
规格：25 reactions
价格：6167
产品类型：蛋白凝胶电泳
品牌：Thermo Fisher
| The Thermo Scientific Pierce Albumin/IgG Removal Kit is optimized to decrease the abundant albumin and antibody components of human serum sample in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.This dye/Protein A-based kit uses a mixture of Cibacron Blue Dye and Protein A agarose affinity resin. The dye binds to albumin with relatively good specificity, and Protein A binds to many species and subclasses of IgG. The Thermo Scientific Pierce Albumin/IgG Removal Kit is economical and can be used to treat samples from other species besides human (e.g., monkey, swine, rabbit). The kit is also scalable and sufficient to process 25 samples, each containing 600µg of total serum protein (about 10µL of serum).Low-abundant proteins in human serum and plasma can provide information about human diseases. However, human fluid analysis is often complicated by the presence of high concentrations of albumin and IgG, which can make up more than 70% of total serum protein (Figure, Panel A). The Thermo Scientific Pierce Albumin/IgG Removal Kit uses a classical mixture of Cibacron Blue Dye and Protein A resin for economical removal of albumin and IgG. This kit can process up to 25 samples of approximately 600 µg of serum using an optimized protocol to minimize nonspecific protein interactions and sample dilution (Figure, Panel C). |
| 特点： |
| - Optimized for 2D sample prep—microcentrifuge spin cup method is scaled and optimized for treating 10 µL human serum samples for 2D electrophoresis- Efficient—kit removes nearly all HSA and IgG, enabling low-abundance proteins to be detected in 2D gels- Fast—kit processes samples in less than 40 minutes- Scalable—use different amounts of affinity resin to match sample volume and concentration |
| 数据： |
2D gels of unprocessed vs processed human serumApproximately 30 µg of unprocessed human serum was isoelectric-focused on a pH 5-8 strip and then separated in the second dimension on an 8-16% SDS polyacrylamide gel. The Pierce Silver Stain Kit (Part No. 24612) was used to visualize bands.Panel A. Unprocessed serum. Large poorly resolved bands corresponding to human serum and IgG obscure much of the gel.Panel B. Processed with the Pierce Antibody-Based Albumin/IgG Removal Kit. With albumin and IgG removed, low-abundant proteins are readily identified.Panel C. Processed with the Pierce Albumin/IgG Removal Kit (dye- and Protein A-based). With albumin and IgG removed, low-abundant proteins are readily identified. |
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