CD54 (ICAM-1) Monoclonal Antibody (HA58), Functional Grade, eBioscience™/CD54 (ICAM-1) 单克隆 (HA58), 功能抗体, eBioscience™

货号：16-0549-82,16-0549-38,16-0549-025,16-0549-050

规格：100 µg,5 mg,25 mg,50 mg

价格：1992,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD54

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：HA58

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）;Assay-Dependent（对照）;Assay-Dependent（功能检测）

产品详细信息Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis. HA58 has also been reported in blocking ofin vitro mixed lymphocyte reaction.Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.仅用于科研。不用于诊断过程。

数据

CD54 (ICAM-1) Antibody (16-0549-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD54 (ICAM-1) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

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参考文献：

流式1.PloS oneEffect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Flow Cytometry"2.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismNeuroprotective effects of mesenchymal stem cells derived from human embryonic stem cells in transient focal cerebral ischemia in rats."16-0549 was used in Flow cytometry/Cell sorting to show intravenously administered embryonic mesenchymal stem cells differentiated into neuronal and endothelial cells, and were neuroprotective in transient focal cerebral ischemia."WB3.Nature communicationsThe critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in ChIP assay"IF4.TheranosticsCD54-NOTCH1 axis controls tumor initiation and cancer stem cell functions in human prostate cancer."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0549-82) in Immunofluorescence"IHC5.Proceedings of the National Academy of Sciences of the United States of AmericaThe human natural killer cell immune synapse.AuthorsDavis DM,Chiu I,Fassett M,Cohen GB,Mandelboim O,Strominger JL

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