5'-DMT-G(dmf)-Suc-CPG; 500 Å (RNA)
货号：BG5-1200FR-1
规格：1 g
价格：2814
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| 5'-DMT-G(DMF)-Suc-RNA CPG is used for the placement of G on the 3' end of oligoribonucleotides. . This support has a 2’ acetyl protecting group which is easily cleaved during deprotection. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. The use of this 5’-DMT protected RNA support is identical to a DNA support with the same protection groups. |
| 用途： |
| - Synthesis conditions: Follow the instrument manufacturer's protocol when using this product.- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C when using fast deprotecting amidites.
We do not recommend using AMA to cleave or deprotect sequences that contain uridine. The AMA may degrade the uridine nucleobase.- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 345.2 |
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