2'-O-Methyl-G(iBu)-Suc-CPG; 500 Å (RNA)
货号：BG5-1200MR-1
规格：1 g
价格：3940
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| 2’-O-Methyl-G(iBu)-Suc-CPG is used for synthesis of oligonucleotides that contain nuclease resistant 2’-O-methyl linkages to prevent degradation. The 2’-O-Methyl-G(iBu)-Suc-CPG requires no special handling or deprotection reagents. The 500 Å CPG is best suited for the synthesis of RNA sequences up to 50-mers in length. |
| 特性： |
| - Appearance: White Powder |
| 用途： |
| - Synthesis conditions:Follow the instrument manufacturer's protocol when using this product.- Cleavage conditions:Use concentrated ammonia for 90 minutes at 25°C or for 30 minutes at 60°C. When using fast deprotecting amidites( CAc; GDMF) 1:1 ammonia:methylamine (AMA) for 25 minutes at 25°C may also be used.- Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF) use concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated ammonia for 5 hours at 60 °C.
Increase time for deprotection if there is an increase in the GiBu content of the oligo.
This RNA support is compatible with deprotection chemistries used with 2’ Fluoro; 2' modified phosphoramidites and TBDMS protected reagents.AMA is not recommended for sequences containing uridine bases. Image of cleaved and deprotected structure: - The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 359.23 |
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