Estrone-3-Sulfate (E1S) Competitive ELISA Kit/硫酸雌酮3（E1S）竞争性ELISA试剂盒

货号：EIA17E3S,EIA17E3SX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	26.4 pg/mL
检测范围	40.96-4000 pg/mL
样本类型/体积	粪便提取物 50 µL 血浆 1 µL 血清 1 µL 上清液 50 µL 尿液 6.25 µL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	8.5%
批内变异系数（CV）	4.2%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Estrone-3-Sulfate
Protein aliases	C18H22O5S, Estrone sulfate
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Human Estrone-3 Sulfate (E1S) ELISA quantitates human estrone-3 Sulfate in human serum, plasma, urine, dried fecal extracts or cell culture medium.人雌酮-3-硫酸盐（E1S）ELISA定量检测人血清，血浆，尿液，干燥的粪便提取物或细胞培养基中的人雌酮3硫酸盐。Principle of the method 方法原理The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices, including serum, plasma, urine, and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.雌酮-3-硫酸盐（E1S）竞争性ELISA试剂盒使用专门生成的抗体来测量多种基质（包括血清，血浆，尿液和粪便）中的Estrone-3-Sulfate（E1S）。该试剂盒将定量测量稀释的缓冲液样品和组织培养基样品中存在的E1S。提供E1S标准以生成测定的标准曲线，所有样品应从标准曲线上读出。将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获兔抗体。将E1S-过氧化物酶结合物添加到孔中的标准品和样品中。通过向每个孔中添加针对E1S的多克隆抗体来启动结合反应。温育2小时后，洗涤板并加入底物。底物与结合的E1S-过氧化物酶偶联物反应。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Estrone-3-sulfate (1, 3, 5(10)-Estratrien-3-ol-17-one sulfate (E1S) is synthesized in the fetal or cotyledonary portion of the placentome. Estrone sulfate, present in plasma in a higher concentration than either unconjugated estrone or estradiol in nonpregnant women and normal men, appears to originate almost entirely from a conjugation of estrone and converted estradiolin nonglandular tissues. Estrone sulfate is only slowly cleared from plasma, thus its concentration does not fluctuate markedly during the day. Estrone sulfate is quantitatively the most important circulating estrogen. Estrone-3-sulfate (E1S) is identical across all species and so this kit should measure estrone-3-sulfate from all sources.雌酮-3-硫酸盐（1、3、5（10）-雌三烯-3-醇-17-硫酸盐（E1S）是在胎盘的胎儿或子叶部分合成的。硫酸雌酮以较高的浓度存在于血浆中 与未结合的雌酮或雌二醇相比，在未怀孕的妇女和正常男性中，雌激素和雌二醇的转化几乎几乎完全源自雌激素和转化的雌二醇非腺组织的结合，硫酸雌酮仅从血浆中缓慢清除，因此其浓度在白天不会明显波动。 硫酸盐在数量上是最重要的循环雌激素，雌酮-3-硫酸盐（E1S）在所有物种中都是相同的，因此该试剂盒应测量所有来源的雌酮3-硫酸盐。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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