IGFBP-5 Mouse ELISA Kit/小鼠IGFBP-5 ELISA试剂盒
货号：EMIGFBP5,EMIGFBP5X5,EMIGFBP5X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 5 pg/mL |
| 检测范围 | 4.12-3000 pg/mL |
样本类型/体积 | 血清 1 μL 血浆 1 μL 上清液 100 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IGFBP5 |
| Protein aliases | IBP-5, IGF-binding protein 5, IGFBP-5, Insulin-like growth factor-binding protein 5 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AI256729, AW208790, IGFBP-5, IGFBP-5P, Igfbp5 |
| Gene ID | (Mouse) 16011 |
| Gene symbol | Igfbp5 |
| UniProt ID | (Mouse) Q07079 |
| The Mouse IGF Binding Protein 5 (Ms IGFBP-5) ELISA quantitates Ms IGFBP-5 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IGFBP-5.小鼠IGF结合蛋白5（Ms IGFBP-5）ELISA定量了小鼠血清，血浆或细胞培养基中的Ms IGFBP-5。 该检测试剂盒将专门识别天然和重组Ms IGFBP-5。Principle of the method 方法原理The Mouse IGFBP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IGFBP-5固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The insulin-like growth factor-binding proteins, or IGFBPs, are a family of homologous proteins that have co-evolved with the IGFs. They serve not only as shuttle molecules for the soluble IGFs, but also confer a level of regulation to the IGF signaling system. Physical association of the IGFBPs with IGF influences the bio-availability of the growth factors, as well as their concentration and distribution in the extracellular environment. In addition, the IGFBPs appear to have biological activity independent of the IGFs. Seven IGFBPs have thus far been described, each differing in their tissue distribution, half-lives and modulation of IGF interactions with their receptors. For instance, IGFBP1 is negatively regulated by insulin production. The IGFBP1 gene is expressed at a high level during fetal liver development and in response to nutritional changes and diabetes. It has been suggested that IGFBP2 functions as chaperone, escorting IGFs to their target tissues. It is expressed in several human tissues including fetal eye and fetal brain. IGFBP3 is the most abundant IGFBP and is complexed with roughly 80% of the serum IGFs. Both IGFBP3 and IGFBP4 are released by dermal fibroblasts in response to incision injury. IGFBP5 is secreted by myoblasts and may play a key role in muscle differentiation.胰岛素样生长因子结合蛋白或IGFBP是与IGF共同进化的同源蛋白家族。它们不仅充当可溶性IGF的穿梭分子，而且还赋予IGF信号传导系统一定水平的调节。 IGFBP与IGF的物理结合会影响生长因子的生物利用度，以及它们在细胞外环境中的浓度和分布。另外，IGFBPs似乎具有独立于IGFs的生物学活性。迄今为止，已经描述了七个IGFBP，每个IGFBP的组织分布，半衰期以及对IGF与受体的相互作用的调节都不同。例如，IGFBP1受胰岛素产生负调节。在胎儿肝脏发育过程中以及对营养变化和糖尿病的反应中，IGFBP1基因高水平表达。已经建议IGFBP2起分子伴侣的作用，将IGFs护送到其靶组织。它在包括胎儿眼和胎儿脑在内的几种人类组织中表达。 IGFBP3是最丰富的IGFBP，并与大约80％的血清IGFs复合。皮肤IGFBP3和IGFBP4均由真皮成纤维细胞释放，以响应切口损伤。 IGFBP5由成肌细胞分泌，可能在肌肉分化中起关键作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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