Myeloperoxidase Mouse ELISA Kit/小鼠髓过氧化物酶ELISA试剂盒
货号：EMMPO,EMMPOX5,EMMPOX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 600 pg/mL |
| 检测范围 | 0.61-150 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Myeloperoxidase |
| Protein aliases | MPO, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | mKIAA4033, Mpo |
| Gene ID | (Mouse) 17523 |
| Gene symbol | Mpo |
| UniProt ID | (Mouse) P11247 |
| The Mouse Myeloperoxidase ELISA quantitates Ms Myeloperoxidase in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Myeloperoxidase.小鼠髓过氧化物酶ELISA可以定量小鼠血清，血浆或细胞培养基中的髓过氧化物酶。 该检测试剂盒将专门识别天然和重组Ms髓过氧化物酶。Principle of the method 方法原理The Mouse Myeloperoxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠髓过氧化物酶固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains with a molecular weight range of 59 - 64 kDa, and two unglycosylated beta chains (MW ~14 kDa) with total MW ~150 kDa and a theoretical pI of 9.2. Traditionally, myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities such as periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently, it was shown that myeloperoxidase participates in the initiation and progression of cardiovascular disease. Myeloperoxidase possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. Myeloperoxidase is part of the host defense system of polymorphonuclear leukocytes and myeloperoxidase is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN (polymorphonuclear leukocytes), MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.髓过氧化物酶（MPO）是在嗜中性粒细胞中大量表达并在其活化过程中分泌的血蛋白。天然髓过氧化物酶表示为两条分子量为59-64 kDa的糖基化α链和两条总MW约为150 kDa且理论pI为9.2的未糖基化的β链（MW〜14 kDa）的共价结合四聚体复合物。传统上，髓过氧化物酶被认为是抗中性粒细胞胞质抗体（ANCA）的主要靶标，ANCA是某些系统性血管（如结节性大动脉炎，显微多动脉炎和肺嗜酸性粒细胞瘤（Churg-Strauss综合征））的血清学标志物。类风湿关节炎也有低至中等的抗髓过氧化物酶自身抗体水平报道。最近，显示髓过氧化物酶参与心血管疾病的发生和发展。髓过氧化物酶具有有效的促炎特性，可能直接导致组织损伤。现在，髓过氧化物酶被认为是最有前途的心脏标志物之一。髓过氧化物酶是多形核白细胞宿主防御系统的一部分，而髓过氧化物酶负责针对多种生物的杀微生物活性。在刺激的PMN（多形核白细胞）中，MPO催化次卤酸（在生理情况下主要为次氯酸）和其他有毒中间体的产生，这些中间体极大地增强了PMN的杀微生物活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20404.htm
QQ:1749072012