DAN (PARN) Mouse ELISA Kit/小鼠DAN (PARN) ELISA试剂盒
货号：EMPARN,EMPARNX5,EMPARNX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 35 pg/mL |
| 检测范围 | 40.96-10,000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | PARN |
| Protein aliases | Poly(A)-specific ribonuclease PARN, Polyadenylate-specific ribonuclease |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | 1200003I18Rik, DAN, Parn |
| Gene ID | (Mouse) 74108 |
| Gene symbol | Parn |
| UniProt ID | (Mouse) Q8VDG3 |
| The Mouse DAN (PARN) ELISA quantitates Ms DAN in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DAN.小鼠DAN（PARN）ELISA可以定量检测小鼠血清，血浆或细胞培养基中的DAN。 该检测试剂盒将专门识别天然和重组DAN。Principle of the method 方法原理The Mouse DAN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠DAN固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.该基因编码的蛋白质是3'-外切核酸酶，与3'-外切核酸酶的RNase D家族相似。 它更喜欢使用poly（A）作为底物，因此可以有效降解mRNA的poly（A）尾巴。 poly（A）尾部的核酸外降解通常是真核mRNA衰减的第一步。 该蛋白还涉及卵母细胞成熟和早期胚胎发育过程中某些母体mRNA的沉默，以及包含早熟终止密码子的mRNA的无义介导的衰变（NMD）。 已经发现该基因的编码不同同工型的剪接的转录变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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