6Ckine/CCL21 Human ELISA Kit/人6Ckine/CCL21 ELISA试剂盒
货号：EHCCL21,EHCCL21X5,EHCCL21X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 32.76-8000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CCL21 |
| Protein aliases | 6Ckine, beta chemokine exodus-2, Beta-chemokine exodus-2, C-C motif chemokine 21, chemokine (C-C motif) ligand 21, Efficient Chemoattractant for Lymphocytes, secondary lymphoid tissue chemokine, Secondary lymphoid-tissue chemokine, SLC, small inducible cytokine subfamily A (Cys-Cys), member 21, Small-inducible cytokine A21 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | 6Ckine, CCL21, CKb9, ECL, SCYA21, SLC, TCA4, UNQ784/PRO1600 |
| Gene ID | (Human) 6366 |
| Gene symbol | CCL21 |
| UniProt ID | (Human) O00585 |
| The Human 6Ckine/CCL21 ELISA quantitates Hu 6Ckine in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu 6Ckine.人6Ckine / CCL21 ELISA可以定量人血清，血浆或细胞培养基中的胡6Ckine。 该检测试剂盒将专门识别天然和重组Hu 6Ckine。Principle of the method 方法原理The Human 6Ckine solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人6Ckine固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).该基因是簇集在9号染色体p臂上的几个CC细胞因子基因之一。细胞因子是涉及免疫调节和炎症过程的一系列分泌蛋白。 CC细胞因子是特征在于两个相邻半胱氨酸的蛋白质。 与其他趋化因子类似，该基因编码的蛋白质抑制造血作用并刺激趋化性。 该蛋白对胸腺细胞和活化的T细胞具有体外趋化作用，但对B细胞，巨噬细胞或嗜中性粒细胞则无趋化作用。 该基因编码的细胞因子也可能在介导淋巴细胞向次级淋巴器官的归巢中起作用。 它是趋化因子受体7（CCR7）的高亲和力功能性配体，在T和B淋巴细胞上表达，并且是细胞因子家族另一个成员（小诱导型细胞因子A19）的已知受体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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