CD163 Human ELISA Kit/人CD163 ELISA试剂盒
货号：EHCD163,EHCD163X5,EHCD163X10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 32.77-8000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 2 μL 血浆 2 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CD163 |
| Protein aliases | CD163, Hemoglobin scavenger receptor, sCD163, macrophage-associated antigen, Scavenger receptor cysteine-rich type 1 protein M130, Soluble CD163 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CD163, M130, MM130, SCARI1 |
| Gene ID | (Human) 9332 |
| Gene symbol | CD163 |
| UniProt ID | (Human) Q86VB7 |
| The Human CD163 ELISA quantitates Hu CD163 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD163.人CD163 ELISA定量人血清，血浆或细胞培养基中的Hu CD163。 该检测试剂盒将专门识别天然和重组Hu CD163。Principle of the method 方法原理The Human CD163 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CD163固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.CD163（M130抗原，Ber-Mac3，Ki-M8，SM4）是一个130 kDa的膜糖蛋白，是富含清除剂受体的半胱氨酸超家族的成员，也是血红蛋白-触珠蛋白复合物的受体。 CD163保护组织免受游离的血红蛋白介导的氧化损伤，并可能通过血红蛋白/触珠蛋白的内吞作用和随后的血红素分解在铁的吸收和循环中发挥作用。 CD163仅在主要在炎症晚期发展的人单核细胞和巨噬细胞的细胞表面表达。具体而言，CD163存在于所有循环单核细胞和大多数组织巨噬细胞上，除了在淋巴滤泡的披覆区和生发中心，指状网状细胞和朗格汉氏细胞中发现的那些。 CD163存在于所有CD14阳性单核细胞，大多数CD64阳性单核细胞上，并在CD16阳性单核细胞上显示出更高的表达。 IL-10，IL-6和地塞米松在单核吞噬细胞上表达CD163。脂多糖（LPS）和肉豆蔻酸乙酸佛波酯（PMA）都诱导CD163从细胞表面脱落到血浆或细胞上清液中。 CD163以钙依赖和pH依赖的方式结合血红蛋白/触珠蛋白复合物，并且对血红蛋白和HP1F表型的多聚触觉蛋白的复合物表现出比对HP1S表型的血红蛋白和二聚触珠蛋白的复合物更高的亲和力。此外，CD163还诱导细胞内信号的级联反应，该信号涉及酪氨酸激酶依赖性钙动员，肌醇三磷酸的产生以及IL6和CSF1的分泌。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20409.htm
QQ:1749072012