NGF beta Human ELISA Kit/人NGF β ELISA试剂盒
货号：EHNGF,EHNGFX5,EHNGFX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 14 pg/mL |
| 检测范围 | 6.86-5000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | NGF |
| Protein aliases | Beta-nerve growth factor, Beta-NGF, nerve growth factor (beta polypeptide), nerve growth factor, beta subunit |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Beta-NGF, HSAN5, NGF, NGFB |
| Gene ID | (Human) 4803 |
| Gene symbol | NGF |
| UniProt ID | (Human) P01138 |
| The Human Nerve Growth Factor beta(Hu NGFβ) ELISA quantitates Hu NGFβ in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NGFβ.人神经生长因子β（人NGFβ）ELISA定量检测人血清，血浆或细胞培养基中的人NGFβ。 该检测试剂盒将只识别天然和重组人NGFβ。Principle of the method 方法原理The Human NGFβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人NGFβ固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.神经生长因子（NGF）影响各种神经元和非神经元细胞的存活和分化。该多肽的作用是由于与特异性细胞表面受体结合而产生的，这些受体以高亲和力和低亲和力位点的形式存在。尽管两种受体都结合NGF，但只有高亲和力形式能够进行信号转导。用125 I-NGF进行的交联研究表明，大鼠PC12细胞的高亲和力受体与NGF形成Mr 158,000复合物，而低亲和力受体形成Mr 100,000复合物。人和大鼠NGF受体基因的全长cDNA克隆已产生并测序，揭示出这两个物种之间的同源性超过90％。 NGF表现出神经生长刺激活性，并参与调节交感神经和某些感觉神经元的生长和分化。此外，NGF刺激交感和胚胎感觉神经元的分裂和分化。 NGF基因的突变与遗传性感觉神经和自主神经病5型（HSAN5）有关，而NGF基因表达失调与变应性鼻炎有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20419.htm
QQ:1749072012