TSLP Human ELISA Kit/人TSLP ELISA试剂盒

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TSLP Human ELISA Kit/人TSLP ELISA试剂盒

货号:EHTSLP,EHTSLPX5,EHTSLPX10

规格:96 tests,5X96 tests,10X96 tests

价格:7311,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

3 pg/mL

检测范围3.28-800 pg/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameTSLP
Protein aliasesThymic stromal lymphopoietin

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesTSLP
Gene ID(Human) 85480
Gene symbolTSLP
UniProt ID

(Human) Q969D9

The Human TSLP ELISA quantitates Hu TSLP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TSLP.人TSLP ELISA可定量人血清,血浆或细胞培养基中的人TSLP。 该检测试剂盒将专门识别天然和重组人TSLP。Principle of the method 方法原理The Human TSLP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TSLP固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.TSLP是一种159个氨基酸的四螺旋束造血细胞因子,可通过由人TSLP受体和IL-7Rα链组成的异二聚体受体复合物发出信号。 它影响髓样细胞并诱导单核细胞释放吸引T细胞的趋化因子,增强CD11c(+)树突状细胞,幼稚CD4(+)和CD8(+)T细胞成熟至变态反应性效应子,并引发树突状细胞成熟 CD40配体刺激后产生大量IL-12。 TSLP影响调节性T细胞阳性选择的调节和外周CD4(+)T细胞稳态的维持。 它通过直接激活肥大细胞诱导过敏性炎症,并参与炎症性关节炎的病理生理。 它也在心脏,肝脏和前列腺中表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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