DNA Damage Competitive ELISA Kit/DNA Damage竞争性ELISA试剂盒
货号：EIADNAD,EIADNADX10
规格：96 tests,10X96 tests
价格：8792,64531
产品类型：ELISA检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 50.9 pg/mL |
| 检测范围 | 62.6-8000 pg/mL |
样本类型/体积 | 消化的DNA 50 µL 粪便提取物 50 µL 血浆 6.25 µL 唾液 25 µL 血清 6.25 µL 上清液 50 µL 尿液 12.5 µL |
操作时间 | 1 hr 20 min |
测试时间 | 2 hr 30 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 9.9% |
| 批内变异系数（CV） | 9% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 测定稀释浓缩液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | -20°C |
| Protein name | DNA Damage |
试剂盒类型 | Competitive ELISA |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| The DNA Damage ELISA quantitates PGFM in serum, plasma, saliva, urine, digested DNA, fecal extracts, or cell culture medium.DNA损伤ELISA可定量测定血清，血浆，唾液，尿液，消化的DNA，粪便提取物或细胞培养基中的PGFM。Principle of the method 方法原理The DNA Damage Competitive ELISA research-use-only kit detects all three oxidized guanine species; 8-hydroxy-2’- deoxyguanosine from DNA, 8-hydroxyguanosine from RNA, and 8-hydroxyguanine from digested DNA from DNA or RNA. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples. An 8-hydroxy-2’-deoxyguanosine (8-OHdG) stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An 8-hydroxyguanosine conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a peroxidase-labeled mouse monoclonal antibody to 8-hydroxy-2’-deoxyguanosine to each well. After a 1-hour incubation the plate is washed and substrate added. The substrate reacts with the peroxidase labeled antibody that has reacted with the bound conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.DNA损伤竞争性ELISA专用研究试剂盒可检测所有三种氧化鸟嘌呤种类； DNA中的8-羟基-2'-脱氧鸟苷，RNA中的8-羟基鸟苷和DNA或RNA中消化的DNA的8-羟基鸟嘌呤。这些物质可能存在于血清，血浆，唾液，尿液，干燥的粪便样本和组织培养基样本中。提供了8-羟基-2'-脱氧鸟苷（8-OHdG）储备液以生成测定的标准曲线，所有样品均应从标准曲线上读出。将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获兔抗体。将8-羟基鸟苷共轭物添加到孔中的标准品和样品中。通过将过氧化物酶标记的小鼠单克隆抗体添加到每个孔的8-hydroxy-2'-deoxyguanosine中来启动结合反应。温育1小时后，洗涤板并加入底物。底物与已经与结合的偶联物反应的过氧化物酶标记的抗体反应。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nucleic acids, proteins, and lipids. Intracellular free radical species (ROS) are produced as a result of normal metabolism and extracellular forms are produced as a result of ultraviolet radiation or ionizing radiation. Cellular function may be interrupted or stopped if DNA damage corrupts the integrity of essential information contained in the genome. When individual bases are damaged, nonspecific DNA repair enzymes excise DNA lesions to release deoxynucleotides, and base specific repair glycosylases excise the corresponding base. Deoxynucleotides are enzymatically hydrolyzed to stable deoxynucleosides, and these repair products are transported through the blood and excreted in the urine. Damage to RNA is reflected in nucleoside adducts. Among numerous types of oxidative DNA damage, the formation of 8-hydroxy-2’-deoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG is physiologically formed and enhanced by chemical carcinogens. During the repair of damaged DNA in vivo by exonucleases, the resulting 8-OHdG is excreted without further metabolism into urine. 8-Hydroxy-2’-deoxyguanosine (8-OHdG) is identical across all species.自由基和其他反应性物种在体内不断产生，对生物分子造成氧化损伤，只有通过多种抗氧化剂和修复系统的存在以及受损核酸，蛋白质和脂质的替代，才能制止这一过程。正常代谢会产生细胞内自由基（ROS），紫外线或电离辐射会产生细胞外形式。如果DNA损伤破坏了基因组中必需信息的完整性，则细胞功能可能会中断或停止。当单个碱基受损时，非特异性DNA修复酶会切除DNA损伤以释放脱氧核苷酸，而碱基特异性修复糖基化酶则会切除相应的碱基。脱氧核苷酸被酶水解为稳定的脱氧核苷，这些修复产物通过血液运输并在尿液中排泄。 RNA的损害反映在核苷加合物中。在许多类型的氧化性DNA损伤中，8-羟基-2'-脱氧鸟苷（8-OHdG）的形成是氧化应激的普遍标志。 8-OHdG在生理上由化学致癌物形成并增强。在通过核酸外切酶在体内修复受损的DNA的过程中，所产生的8-OHdG会被排泄，而不会进一步代谢为尿液。所有物种中的8-Hydroxy-2'-deoxyguanosine（8-OHdG）均相同。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。authorization. |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20424.htm
QQ:1749072012