Insulin Rat ELISA Kit/大鼠胰岛素ELISA试剂盒
货号：ERINS,ERINSX5
规格：96 tests,5X96 tests
价格：7311,29891
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 5 µIU/mL |
| 检测范围 | 4.69-300 µIU/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Insulin |
| Protein aliases | Insulin-1, Insulin-1 A chain, Insulin-1 B chain, Insulin-2, Insulin-2 A chain, Insulin-2 B chain |
物种（Tested） | 大鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Ins-1, Ins-2, Ins1, Ins2 |
| Gene ID | (Rat) 24505,(Rat) 24506 |
| Gene symbol | Ins1, Ins2 |
| UniProt ID | (Rat) P01322,(Rat) P01323 |
| The Rat Insulin ELISA quantitates Rt Insulin in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Insulin.大鼠胰岛素ELISA可以定量大鼠血清，血浆或细胞培养基中的大鼠胰岛素。 该检测试剂盒将只识别天然和重组大鼠胰岛素。Principle of the method 方法原理The Rat Insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠胰岛素固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.胰岛素是胰岛β细胞分泌的一种肽激素。 通过增强葡萄糖，氨基酸和某些离子的膜运输来调节碳水化合物，蛋白质和脂质的代谢。 还促进糖原的储存，甘油三酸酯的形成以及蛋白质和核酸的合成。 胰岛素是由前体肽胰岛素原产生的51个氨基酸的多肽产物。 胰岛素在翻译后被切割成两条链（肽A和肽B），它们通过两个二硫键和一个C肽分子共价连接。 胰岛素缺乏会导致糖尿病，这是普通人群发病和死亡的主要原因之一。 胰岛素还存在于b细胞起源的肿瘤（例如胰岛素瘤）中。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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