Galectin-3 (LGALS3) Rat ELISA Kit/大鼠Galectin-3 (LGALS3) ELISA试剂盒

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Galectin-3 (LGALS3) Rat ELISA Kit/大鼠Galectin-3 (LGALS3) ELISA试剂盒

货号:ERLGALS3,ERLGALS3X5

规格:96 tests,5X96 tests

价格:7311,29891

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:大鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

3 pg/mL

检测范围3.28-800 pg/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameGalectin 3
Protein aliases35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, epsilon BP, Gal-3, Galactose-specific lectin 3, Galectin-3, IgE binding protein, IgE-binding protein, Laminin-binding protein, lectin galactoside-binding soluble 3 protein, Lectin L-29, lectin, galactose binding, soluble 3, Mac-2 antigen

物种(Tested)

大鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesAGE-R3, CBP30, gal-3, L-34, Lgals3
Gene ID(Rat) 83781
Gene symbolLgals3
UniProt ID

(Rat) P08699

The Rat Galectin-3 (LGALS3) ELISA quantitates Rt Galectin-3 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Galectin-3.大鼠Galectin-3(LGALS3)ELISA定量了大鼠血清,血浆或细胞培养基中的大鼠Galectin-3。 该检测试剂盒将专门识别天然和重组大鼠Galectin-3。Principle of the method 方法原理The Rat Galectin-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠Galectin-3固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.结合IgE的半乳糖特异性凝集素。 介导α-3,β-1整联蛋白通过CSPG4刺激内皮细胞迁移。 与DMBT1一起,是早期胚胎发生过程中柱状上皮细胞终末分化所必需的。 在细胞核中:充当前mRNA剪接因子。 涉及急性炎症反应,包括嗜中性粒细胞活化和粘附,单核细胞巨噬细胞的化学引诱,凋亡性嗜中性粒细胞的调理作用以及肥大细胞的活化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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