IL-12 (IL-12A) Porcine ELISA Kit/猪IL-12 (IL-12A) ELISA试剂盒
货号:ESIL12A,ESIL12AX5
规格:96 tests,5X96 tests
价格:7238,29891
产品类型:Platinum ELISA
品牌:Thermo Fisher
物种:其它
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | 2.2 ng/mL |
检测范围 | 2.458-600 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
批间变异系数(CV) | <12% |
批内变异系数(CV) | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
Protein name | IL-12 p35 |
Protein aliases | CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12A, IL-12B, interleukin 12 p35 subunit, interleukin-12 p40 subunit, Interleukin-12 subunit alpha, Interleukin-12 subunit beta |
物种(Tested) | 猪 |
试剂盒类型 | Sandwich ELISA Kit |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene aliases | IL12A, IL12B |
Gene ID | (Pig) 397053,(Pig) 397076 |
Gene symbol | IL12A, IL12B |
UniProt ID | (Pig) Q29053,(Pig) Q28938 |
The Porcine Interleukin 12 (IL-12A) ELISA quantitates Porc IL-12A in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-12A.猪白介素12(IL-12A)ELISA定量猪血清,血浆或细胞培养基中的猪IL-12A。 该检测试剂盒将专门识别天然和重组猪IL-12A。Principle of the method 方法原理The Porcine IL-12A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.猪IL-12A固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) encoded by the IL12B gene and 35-kD (p35) encoded by the IL12A gene. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.白介素12(IL-12)是一种异源二聚体70 kD(p70)细胞因子,由两条共价连接的糖基化链组成,其中IL12B基因编码的40kD(p40)和IL12A基因编码的35-kD(p35)。 IL-12主要由单核细胞,巨噬细胞和树突状细胞响应细菌产品(如脂多糖(LPS)),细胞内病原体或与活化T细胞相互作用而产生。 最初发现IL-12是因为它具有诱导干扰素-γ(IFN-γ)产生,细胞增殖以及由自然杀伤细胞和T细胞介导的细胞毒性的能力。 现在已经确定,IL-12在Th1反应的发展中也起着关键作用,导致IFN-γ和IL-2的产生。 这些细胞因子可进而促进T细胞反应和巨噬细胞活化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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