HTRA2 (OMI) Human ELISA Kit/人HTRA2 (OMI) ELISA试剂盒
货号：EHHTRA2,EHHTRA2X5,EHHTRA2X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 200 pg/mL |
| 检测范围 | 0.205-50 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | HTRA2 |
| Protein aliases | High temperature requirement protein A2, HtrA-like serine protease, HtrA2, Omi stress-regulated endoprotease, protease, serine, 25, Serine protease 25, Serine protease HTRA2, mitochondrial, Serine proteinase OMI |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | HTRA2, OMI, PARK13, PRSS25 |
| Gene ID | (Human) 27429 |
| Gene symbol | HTRA2 |
| UniProt ID | (Human) O43464 |
| The Human HTRA2(OMI) ELISA quantitates Hu OMI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu OMI.人HTRA2（OMI）ELISA可以定量人类血清，血浆或细胞培养基中的人OMI。 该检测试剂盒将专门识别天然和重组的人OMI。Principle of the method 方法原理The Human OMI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人OMI固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP (4). Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.蛋白质的凋亡抑制剂（IAP）家族调节由各种刺激触发的程序性细胞死亡。 所有IAP具有至少一个杆状病毒IAP重复（BIR）基序，这对于其抗凋亡活性至关重要（1-3）。 最近，已经分离出丝氨酸蛋白酶，其在凋亡刺激诱导的细胞凋亡后从线粒体释放。 这种称为Omi / HtrA2的蛋白质是与IAP结合的线粒体蛋白质（4）。 像Smac / DIABLO一样，成熟的Omi蛋白在其N末端包含一个保守的IAP结合基序（AVPS）。 Omi在哺乳动物细胞质中的表达失调可诱导细胞凋亡，表明Omi可以参与线粒体的凋亡途径。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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