FGF19 Human ELISA Kit/人FGF19 ELISA试剂盒
货号：EHFGF19,EHFGF19X5,EHFGF19X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 32.77-8000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | FGF19 |
| Protein aliases | FGF-19, Fibroblast growth factor 19 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | FGF19, UNQ334/PRO533 |
| Gene ID | (Human) 9965 |
| Gene symbol | FGF19 |
| UniProt ID | (Human) O95750 |
| The Human Fibroblast Growth Factor 19 (Hu FGF19) ELISA quantitates Hu FGF19 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF19.人成纤维细胞生长因子19（Hu FGF19）ELISA定量了人血清，血浆或细胞培养基中的人FGF19。 该检测试剂盒将只识别天然和重组的人FGF19。Principle of the method 方法原理The Human FGF19 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人FGF19固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的目标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.成纤维细胞生长因子1（FGF-1），也称为酸性FGF，和成纤维细胞生长因子2（FGF-2），也称为碱性FGF，是刺激间充质，上皮细胞增殖的生长因子家族的成员。 和神经外胚层起源。 FGF家族的其他成员包括致癌基因FGF-3（Int2）和FGF-4（hst / Kaposi），FGF-5，FGF-6，FGF-7（KGF），FGF-8（AIGF），FGF-9 （GAF）和FGF-10-FGF-23。 FGF家族成员具有30-55％的氨基酸序列同一性和相似的基因结构，并且在转染的细胞中过表达时能够转化培养的细胞。 FGF的细胞受体是第二个多基因家族的成员，该家族包括四个酪氨酸激酶，分别称为Flg（FGFR-1），Bek（FGFR-L），TKF和FGFR-3。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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