Neprilysin (MME) Human ELISA Kit/人脑啡肽酶 (MME) ELISA试剂盒
货号：EHMME,EHMMEX5,EHMMEX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.4 ng/mL |
| 检测范围 | 0.41-100 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CD10 |
| Protein aliases | Atriopeptidase, CALLA, CD10, Common acute lymphocytic leukemia antigen, Enkephalinase, membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CD10), membrane metallo-endopeptidase variant 1, membrane metallo-endopeptidase variant 2, NEP, Neprilysin, neprilysin-390, neprilysin-411, Neutral endopeptidase 24.11, SFE, Skin fibroblast elastase |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CALLA, CD10, CMT2T, EPN, MME, NEP, SFE |
| Gene ID | (Human) 4311 |
| Gene symbol | MME |
| UniProt ID | (Human) P08473 |
| The Human Neprilysin (MME) ELISA quantitates Hu MME in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MME.人脑中性溶素（MME）ELISA定量了人血清，血浆或细胞培养基中的人MME。 该检测试剂盒将专门识别天然和重组人MME。Principle of the method 方法原理The Human MME solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人MME固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CD10 (Common Acute Lymphocytic Leukemia Antigen ,CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.CD10（普通急性淋巴细胞白血病抗原，CALLA）是一种具有中性金属内肽酶活性的细胞表面酶，可以使多种生物活性肽失活。 CD10在成年骨髓的淋巴母细胞，伯基特氏和滤泡生发中心淋巴瘤，未成熟的B细胞以及慢性粒细胞性白血病（CML）患者的细胞上表达。 CD10也存在于乳腺肌上皮细胞，胆小管，成纤维细胞上，在肾脏和肠上皮细胞的刷状缘特别高表达。 CD10是一种中性内肽酶，可在疏水残基的氨基端切割肽，并使几种肽激素失活，包括胰高血糖素，脑啡肽，P物质，神经降压素，催产素和缓激肽。另外，CD10是100kDa的II型跨膜糖蛋白，以大于45kb的单拷贝存在。交替剪接CD10基因的5'非翻译区，产生四个独立的mRNA转录本，而编码区不受剪接的影响。与CD10功能障碍有关的疾病包括脊髓小脑共济失调43和Charcot-Marie牙齿病。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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