Cystatin C (CST3) Mouse ELISA Kit/小鼠胱抑素 C (CST3) ELISA试剂盒
货号：EMCST3,EMCST3X5,EMCST3X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 10 pg/mL |
| 检测范围 | 13.11-3200 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Cystatin C |
| Protein aliases | cystatin 3, Cystatin-3, Cystatin-C |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Cst3, CysC |
| Gene ID | (Mouse) 13010 |
| Gene symbol | Cst3 |
| UniProt ID | (Mouse) P21460 |
| The Mouse Cystatin C (CST3) ELISA quantitates Ms Cystatin C in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Cystatin C.小鼠胱抑素C（CST3）ELISA定量检测小鼠血清，血浆或细胞培养基中的胱抑素C。 该检测试剂盒将专门识别天然和重组Ms 胱抑素C。Principle of the method 方法原理The Mouse Cystatin C solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠半胱氨酸蛋白酶抑制剂C固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Cystatin C is a cysteine (thiol) protease inhibitor that belongs to the type II cystatin gene superfamily and is the most abundant extracellular inhibitor of cysteine proteases. Cystatin C is a constitutively secreted, amyloidogenic protein, which forms a two-fold symmetric dimer and modulates both cysteine protease activity and the expression of class II MHC molecules. Expression of cystatin C is an indicator of kidney function and glomerular filtration rate. Mutations in the cystatin C gene can lead to protein aggregates, which are implicated in hereditary amyloid angiopathy (HCCAA) and cerebral hemorrhage. Although both wild-type and mutant cystatin C are capable of forming concentration dependent inactive dimers, mutant cystatin C dimerizes at lower concentrations and is more susceptible to serine proteases, which may facilitate aggregation. In neuronal cells, oxidative stress stimulates expression of cystatin C, which may positively regulate apoptosis.胱抑素C是一种半胱氨酸（硫醇）蛋白酶抑制剂，属于II型胱抑素基因超家族，是最丰富的半胱氨酸蛋白酶的细胞外抑制剂。 胱抑素C是组成型分泌的淀粉样蛋白，它形成两个对称的二聚体，并调节半胱氨酸蛋白酶的活性和II类MHC分子的表达。 胱抑素C的表达是肾脏功能和肾小球滤过率的指标。 胱抑素C基因的突变可导致蛋白质聚集，这与遗传性淀粉样血管病（HCCAA）和脑出血有关。 尽管野生型和突变型半胱氨酸蛋白酶抑制剂C均能够形成浓度依赖性的非活性二聚体，但突变型半胱氨酸蛋白酶抑制剂C在较低的浓度下二聚，并且对丝氨酸蛋白酶更敏感，这可能有助于聚集。 在神经元细胞中，氧化应激会刺激胱抑素C的表达，这可能会积极调节细胞凋亡。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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