E-Cadherin (CDH1) Mouse ELISA Kit/小鼠E-钙黏着蛋白 (CDH1) ELISA试剂盒
货号：EMCDH1,EMCDH1X5,EMCDH1X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 6 pg/mL |
| 检测范围 | 4.12-3000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | E-cadherin |
| Protein aliases | ARC-1, Epithelial cadherin, E-cadherin, Uvomorulin, Cadherin-1, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AA960649, Cdh1, E-cad, Ecad, L-CAM, Um, UVO |
| Gene ID | (Mouse) 12550 |
| Gene symbol | Cdh1 |
| UniProt ID | (Mouse) P09803 |
| The Mouse E-Cadherin (CDH1) ELISA quantitates Ms E-Cadherin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms E-Cadherin.小鼠E-钙黏着蛋白（CDH1）ELISA定量检测小鼠血清，血浆或细胞培养基中的Ms E-钙黏着蛋白。 该检测试剂盒将专门识别天然和重组Ms E-钙黏着蛋白。Principle of the method 方法原理The Mouse E-Cadherin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠E-钙黏着蛋白固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.E-钙黏着蛋白（上皮钙黏着蛋白）是来自钙黏着蛋白（依赖铝的粘附蛋白）超家族的经典钙黏着蛋白。编码的蛋白质是钙依赖性细胞-细胞粘附糖蛋白，由五个细胞外钙粘蛋白重复序列，跨膜区域和高度保守的细胞质尾部组成。该基因的突变与胃癌，乳腺癌，结肠直肠癌，甲状腺癌和卵巢癌有关。人们认为功能丧失通过增加增殖，侵袭和/或转移来促进癌症进展。该蛋白的胞外域介导细菌与哺乳动物细胞的粘附，而胞质域是内在化所必需的。鉴定出的转录物变体来自共有剪接位点处的突变。 E-钙粘着蛋白通过控制组织结构和维持组织完整性，在细胞的生长和发育中起着核心作用。在人类中，E-钙粘蛋白由16号染色体上存在的CDH1基因编码。研究表明，癌中E-钙粘蛋白表达的减少和/或丢失与这些肿瘤侵袭和转移的潜能成正相关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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