VEGFB Mouse ELISA Kit/小鼠VEGFB ELISA试剂盒
货号：EMVEGFB,EMVEGFBX5,EMVEGFBX10
规格：96 tests,5X96 tests,10X96 tests
价格：7311,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.6 ng/mL |
| 检测范围 | 0.655-160 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | VEGFB |
| Protein aliases | Vascular endothelial growth factor B, VEGF-B, VEGF-related factor, VRF |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | VEGF-B, Vegfb, Vrf |
| Gene ID | (Mouse) 22340 |
| Gene symbol | Vegfb |
| UniProt ID | (Mouse) P49766 |
| The Mouse VEGF-B ELISA quantitates Ms VEGF-B in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms VEGF-B.小鼠VEGF-B ELISA可以定量检测小鼠血清，血浆或细胞培养基中的VEGF-Bs。 该检测试剂盒将专门识别天然和重组Ms VEGF-B。Principle of the method 方法原理The Mouse VEGF-B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠VEGF-B固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.血管内皮生长因子（VEGF）是一族紧密相关的生长因子，具有八个半胱氨酸残基的保守模式并共享常见的VEGF受体。 VEGF刺激内皮细胞，诱导血管生成，促进细胞迁移，增加血管通透性并抑制细胞凋亡。 VEGFB与VEGF和PIGF具有结构相似性，并经常与VEGF共表达。 VEGFB有两种剪接的同工型：VEGFB 167和VEGFB186。VEGFB 167是一种高度碱性的肝素结合蛋白，保留在细胞或细胞外基质中，而VEGFB 186则很容易分泌。 VEGFB刺激内皮细胞增殖。 VEGFB与酪氨酸激酶受体VEGFR1（flt1）结合，而不与VEGFR2结合。血管内皮生长因子B广泛表达，但在心脏，骨骼肌和胰腺中含量最高。已经提出，人原发性乳腺癌中的VEGFB表达与淋巴结转移有关。编码VEGFB的基因已定位到11q13号染色体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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