EGF Rat ELISA Kit/大鼠EGF ELISA试剂盒
货号：EREGF,EREGFX5
规格：96 tests,5X96 tests
价格：7238,29826
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.8 pg/mL |
| 检测范围 | 0.82-200 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 25 μL 血浆 25 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | EGF |
| Protein aliases | EGF, Epidermal growth factor, Pro-epidermal growth factor |
物种（Tested） | 大鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Egf |
| Gene ID | (Rat) 25313 |
| Gene symbol | Egf |
| UniProt ID | (Rat) P07522 |
| The Rat Epidermal Growth Factor (Rt EGF) ELISA quantitates Rt EGF in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt EGF.大鼠表皮生长因子（大鼠EGF）ELISA定量了大鼠血清，血浆或细胞培养基中的大鼠EGF。 该检测试剂盒将专门识别天然和重组大鼠EGF。Principle of the method 方法原理The Rat EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠EGF固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息EGF belongs to a family of growth factors that bind to the 170 kDa EGF receptor. The EGF receptor ligands are synthesized as type I transmembrane proteins of 130 kDa with an N-terminal extension called the EGF module and a C-terminal fragment, also known as the cytoplasmic tail. EGF comprises nine EGF motives; only the one adjacent to the cell membrane is functional as an EGF receptor-binding domain. Due to proteolytic cleavage a soluble growth factor of 6 kDa containing the EGF module is released into the extracellular space ('ectodomain shedding'). EGF can be detected in most body fluids; the concentration is especially high in plasma, urine, saliva, and milk. Platelets as well as cells in various organs such as in brain, kidney, salivary gland, and stomach release EGF. As a mitogen for endothelial cells EGF also affects angiogenesis and plays a role in wound healing processes. Blocking the release of EGF receptor ligands inhibits growth and migration in several EGF receptor-dependent cell lines and greatly retards wound re-epithelialization due to impaired keratinocyte migration. Overexpression of one or more receptors and/or ligands is a feature of the majority of human carcinomas and epithelial cancers.EGF属于与170 kDa EGF受体结合的生长因子家族。 EGF受体配体合成为130 kDa的I型跨膜蛋白，其N端延伸称为EGF模块，C端片段也称为胞质尾。 EGF包含9种EGF动机；仅与细胞膜相邻的一个作为EGF受体结合域。由于蛋白水解切割，含有EGF模块的6kDa的可溶性生长因子被释放到细胞外空间（“胞外域脱落”）。在大多数体液中都可以检测到EGF。血浆，尿液，唾液和牛奶中的浓度特别高。各种器官（例如脑，肾，唾液腺和胃）中的血小板以及细胞释放EGF。 EGF作为内皮细胞的促分裂原，也影响血管生成并在伤口愈合过程中发挥作用。阻止EGF受体配体的释放会抑制几种EGF受体依赖性细胞系的生长和迁移，并由于角质形成细胞迁移受损而大大延迟伤口的上皮形成。一种或多种受体和/或配体的过表达是大多数人类癌和上皮癌的特征。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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