IGF-1 Rat ELISA Kit/大鼠IGF-1 ELISA试剂盒
货号：ERIGF1,ERIGF1X5
规格：96 tests,5X96 tests
价格：7238,29826
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 30 pg/mL |
| 检测范围 | 30.72-7500 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | IGF1 |
| Protein aliases | IGF-I, Somatomedin, Insulin-like growth factor I |
物种（Tested） | 大鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Igf-1, Igf1 |
| Gene ID | (Rat) 24482 |
| Gene symbol | Igf1 |
| UniProt ID | (Rat) P08025 |
| The Rat Insulin-Like Growth Factor 1 (Rt IGF-1) ELISA quantitates Rt IGF-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt IGF-1.大鼠胰岛素样生长因子1（大鼠IGF-1）ELISA定量了大鼠血清，血浆或细胞培养基中的大鼠IGF-1。 该检测试剂盒将专门识别天然和重组大鼠IGF-1。Principle of the method 方法原理The Rat IGF-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠IGF-1固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IGF1 (Insulin-like growth factor-1) is structurally and functionally related to insulin but has a much higher growth-promoting activity. A variety of cellular responses are induced by IGF1, including cell proliferation, differentiation, migration, and survival. Further, IGF1 is a polypeptide growth factor that stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue. IGF1 stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regards to enhancing glucose uptake. In circulation, IGFs are predominantly bound to binding proteins (IGFBPs) which prolong the half-life of the IGFs and play a role in delivering them to target tissues. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Moreover, mature human IGF-I is 100% homologous with bovine and porcine proteins. Low levels of IGF1 have been linked to Alzheimer's disease. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in the IGF1 gene are a cause of insulin-like growth factor 1 deficiency and several transcript variants encoding different isoforms have been found.IGF1（胰岛素样生长因子-1）在结构和功能上与胰岛素有关，但具有更高的促生长活性。 IGF1诱导多种细胞应答，包括细胞增殖，分化，迁移和存活。此外，IGF1是一种多肽生长因子，可刺激肌肉，骨骼和软骨组织中多种细胞类型的增殖。 IGF1刺激大鼠骨源性成骨细胞（PyMS）中的葡萄糖转运，在比胰岛素低得多的浓度下有效，不仅在糖原和DNA合成方面，在提高葡萄糖吸收方面。在循环中，IGF主要与结合蛋白（IGFBP）结合，结合蛋白可延长IGF的半衰期，并在将其递送至靶组织中发挥作用。 IGF-1是已知的AKT信号传导途径中最有效的活化剂之一，它是增殖的刺激剂和程序性细胞死亡的抑制剂。此外，成熟的人IGF-1与牛和猪蛋白100％同源。低水平的IGF1与阿尔茨海默氏病有关。 IGF1由前体加工而成，并与特定受体结合并被分泌。 IGF1基因的缺陷是胰岛素样生长因子1缺乏的原因，并且已经发现了几种编码不同同工型的转录变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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