CD25 Monoclonal Antibody (BC96), eBioscience™

货号：14-0259-82,14-0259-95,14-0259-37

规格：100 µg,10 mg,2 mg

价格：1136,28906,11562

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：其它

类型:	一抗	同型对照：	IgG1 kappa
浓度：	1 µg/test	用法：	1 µg/Test（Flow）

产品详细信息Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.<br>Applications Reported: The BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: The BC96 antibody has been tested by flow cytometric analysis of PHA-activated normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.仅用于科研。不用于诊断过程。

数据

CD25 Antibody (14-0259-82) in FlowStaining of PHA stimulated human PBMC with Anti-Human CD25 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. CD25 Antibody (14-0259-82)Experimental and therapeutic medicine 2016 -Figure 1. Flow cytometric mapping of the CD4 + CD25 + Foxp3 + cells in peripheral blood withdrawn from the syphilitic patients with sero-resistance. Foxp3, forkhead box P3.

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参考文献

1.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."Published figure using CD25 monoclonal antibody (Product # 14-0259-82) in Flow Cytometry"2.Investigative ophthalmology and visual scienceT-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma."Published figure using CD25 monoclonal antibody (Product # 14-0259-82) in Flow Cytometry"3.Acta Cardiologica SinicaThe Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis."Published figure using CD25 monoclonal antibody (Product # 14-0259-82) in Flow Cytometry"4.BloodPhosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection."14-0259 was used in Flow cytometry/Cell sorting to assess phosphoantigen-activated V2V2 T-cells for the ability to interplay with Tregs in the context of mycobacterial infection."AuthorsGong G,Shao L,Wang Y,Chen CY,Huang D,Yao S,Zhan X,Sicard H,Wang R,Chen ZW5.Journal of immunology (Baltimore, Md. : 1950)Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells."Published figure using CD25 monoclonal antibody (Product # 14-0259-82) in Flow Cytometry"

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