M-CSF (CSF-1) Human ELISA Kit/人M-CSF (CSF-1) ELISA试剂盒
货号：EHCSF1,EHCSF1X5,EHCSF1X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 5 pg/mL |
| 检测范围 | 2.78-2000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | M-CSF |
| Protein aliases | colony stimulating factor 1 (macrophage), CSF-1, M-CSF, MCSF, Lanimostim, macrophage colony stimulating factor 1, Macrophage colony-stimulating factor 1, Processed macrophage colony-stimulating factor 1 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | CSF-1, CSF1, MCSF |
| Gene ID | (Human) 1435 |
| Gene symbol | CSF1 |
| UniProt ID | (Human) P09603 |
| The Human Macrophage-Colony Stimulating Factor (CSF-1) (Hu M-CSF) ELISA quantitates Hu M-CSF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu M-CSF.人类巨噬细胞集落刺激因子（CSF-1）（Hu M-CSF）ELISA定量了人类血清，血浆或细胞培养基中的Hu M-CSF。 该检测试剂盒将专门识别天然和重组Hu M-CSF。Principle of the method 方法原理The Human M-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人M-CSF固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.M-CSF（巨噬细胞集落刺激因子，CSF-1）是单核细胞，巨噬细胞和破骨细胞祖细胞增殖和发育必不可少的生存因子。 M-CSF还诱导巨噬细胞分泌VEGF（血管内皮生长因子），从而介导内皮祖细胞的动员和新血管形成。 M-CSF以几种生物活性同工型的形式存在，其效力和稳定性各不相同。全长蛋白被合成为跨膜蛋白，该蛋白可以在细胞表面表达或在分泌囊泡中进一步切割和修饰。此外，M-CSF是二硫键结合的同型二聚体，其被加工成两种同工型之一，即糖蛋白或蛋白聚糖，已通过向每个亚基中添加硫酸软骨素而被修饰。 M-CSF与其受体c-Fms（CSF-1R或CD115）的结合诱导受体二聚化，然后复合物内在化和降解。在功能上，已知M-CSF刺激造血干细胞向培养中的单核巨噬细胞细胞群分化。 M-CSF通过CSF受体1起作用。尽管人M-CSF对小鼠细胞显示活性，但小鼠CSF对人细胞没有活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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