NAP-2/PPBP Human ELISA Kit/人NAP-2/PPBP ELISA试剂盒-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

NAP-2/PPBP Human ELISA Kit/人NAP-2/PPBP ELISA试剂盒

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NAP-2/PPBP Human ELISA Kit/人NAP-2/PPBP ELISA试剂盒

货号：EHPPBP,EHPPBPX5,EHPPBPX10

规格：96 tests,5X96 tests,10X96 tests

价格：7238,29826,52892

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	8.5 pg/mL
检测范围	4.1-1000 pg/mL
样本类型/体积	上清液 100 μL 血清 1 μL 血浆 1 μL
操作时间	1 hr 20 min
测试时间	4 hr 45 min
Homogenous (no wash)	No
批间变异系数（CV）	<12%
批内变异系数（CV）	<10%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	PPBP
Protein aliases	Beta-TG, Beta-thromboglobulin, C-X-C motif chemokine 7, chemokine (C-X-C motif) ligand 7, Connective tissue-activating peptide III, Connective tissue-activating peptide III(1-81), CTAP-III, CTAP-III(1-81), CXC chemokine ligand 7, LA-PF4, LDGF, Leukocyte-derived growth factor, Low-affinity platelet factor IV, Macrophage-derived growth factor, MDGF, NAP-2, NAP-2(1-63), NAP-2(1-66), NAP-2(73), NAP-2(74), Neutrophil-activating peptide 2, Neutrophil-activating peptide 2(1-63), Neutrophil-activating peptide 2(1-66), Neutrophil-activating peptide 2(73), Neutrophil-activating peptide 2(74), neutrophil-activating peptide-2, PBP, Platelet basic protein, pro-platelet basic protein (chemokine (C-X-C motif) ligand 7), small inducible cytokine B7, small inducible cytokine subfamily B, member 7, Small-inducible cytokine B7, TC-1, TC-2, thrombocidin 1, thrombocidin 2, thromboglobulin, beta-1
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, PPBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
Gene ID	(Human) 5473
Gene symbol	PPBP
UniProt ID	(Human) P02775

The Human NAP-2/PPBP ELISA quantitates Hu NAP-2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NAP-2.人NAP-2 / PPBP ELISA可以定量人血清，血浆或细胞培养基中的人NAP-2。该检测试剂盒将专门识别天然和重组人NAP-2。Principle of the method 方法原理The Human NAP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人NAP-2固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Members of the a-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif, in which the first two cysteine residues are separated by a single residue (Cys-X-Cys), and are also chemotactic for neutrophils. The platelet basic protein (PBP), a member of the a-chemokine family, resides in the a-granules of platelets and is released upon their activation. Proteolytic cleavage of the amino terminus of PBP leads to the generation of several peptides, which include mature PBP, connective tissue-activating peptide III (CTAP III, also designated low affinity platelet factor IV (LA-PF4)), b-thromboglobulin (b-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12-q13.诱导型，分泌型，促炎性细胞因子的α-趋化因子亚家族的成员具有相似的基序，其中前两个半胱氨酸残基被单个残基（Cys-X-Cys）隔开，并且对中性粒细胞也具有趋化性。血小板碱性蛋白（PBP）是α-趋化因子家族的成员，位于血小板的α-颗粒中，并在其活化后释放。蛋白水解酶对PBP氨基末端的切割导致生成了几种肽，包括成熟的PBP，结缔组织激活肽III（CTAP III，也称为低亲和力血小板因子IV（LA-PF4）），b-血球蛋白（b -TG）和中性粒细胞激活肽2（NAP-2）。 PBP及其N截短的衍生物介导炎症和伤口愈合。具体而言，NAP-2在炎症过程中激活嗜中性粒细胞的趋化性和脱粒作用。编码人PBP的基因定位到4q12-q13染色体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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