产品介绍 |
| Rabbit anti actin beta antibodyrecognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant speciesArabidopsis thaliana(mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target SpeciesCross Reactivity |
| Mouse √ |
| Rat √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography |
AntiserumPreparation | Antiserum to actin beta was raised by repeated immunisation of rabbits with highly purified antigen |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3)0.2% Gelatin |
Immunogen | Peptide mapping to the terminal domain of human actin beta |
Approx. Protein Concentrations | IgG concentration 0.2 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1/200 | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- Rabbit anti actin beta recognizes a band of appriximately 42 kDa when run under reducing conditions
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文献 |
| 1.Chen, X.J.et al.(2018) Association of reduced sclerostin expression with collapse process in patients with osteonecrosis of the femoral head.Int Orthop. 42 (7): 1675-82.2.Savova, M.S.et al.(2021) Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.Biomed Pharmacother. 141: 111934. |
QQ:1749072012