Thyroglobulin Human ELISA Kit/人甲状腺球蛋白ELISA试剂盒

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Thyroglobulin Human ELISA Kit/人甲状腺球蛋白ELISA试剂盒

货号:EHTG,EHTGX5,EHTGX10

规格:96 tests,5X96 tests,10X96 tests

价格:7238,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

220 pg/mL

检测范围0.205-50 ng/mL

样本类型/体积

上清液 100 μL

血清 50 μL

血浆 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameThyroglobulin
Protein aliasesTg, Thyroglobulin

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesAITD3, TG, TGN
Gene ID(Human) 7038
Gene symbolTG
UniProt ID

(Human) P01266

The Human Thyroglobulin (TG) ELISA quantitates Hu TG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TG.人甲状腺球蛋白(TG)ELISA可以定量人血清,血浆或细胞培养基中的人TG。 该检测试剂盒将只识别天然和重组人TG。Principle of the method 方法原理The Human TG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TG固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The human thyroglobulin (hTg) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti-thyroglobulin autoantibodies were the first factor to be discovered. Anti-hTG is found in all thyroid autoimmune diseases (Hashimoto thyroiditis, Graves diseases), with the highest level observed in Hashimoto thyroiditis. Anti-hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients.人甲状腺球蛋白(hTg)是在甲状腺滤泡细胞中发现的一种高分子量糖蛋白(605 kDa)。 它在甲状腺激素T4和T3的摄取,结合和调节生物合成中起着核心作用。 甲状腺疾病在很大程度上归因于自身免疫,抗甲状腺球蛋白自身抗体是第一个被发现的因素。 在所有甲状腺自身免疫性疾病(桥本甲状腺炎,格雷夫斯病)中都发现了抗hTG,在桥本甲状腺炎中发现的水平最高。 抗-hTG也是甲状腺癌的特征,其测定可用于癌症患者的随访。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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