Levonorgestrel (LNG) Competitive ELISA Kit/Levonorgestrel (LNG) 竞争性ELISA试剂盒

货号：EIALNGC,EIALNGCX10

规格：96 tests,10X96 tests

价格：7929,58769

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2.2 pg/mL
检测范围	6.25-200 pg/mL
样本类型/体积	粪便提取物 100 µL 牛奶 100 µL 血浆 100 µL 唾液 50 µL 血清 100 µL 上清液 100 µL 尿液 25 µL 水 100 µL
操作时间	1 hr 20 min
测试时间	1 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	9.1%
批内变异系数（CV）	9%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Levonorgestrel
Protein aliases	C21H28O2
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Levonorgestrel (LNG) ELISA quantitates LNG in serum, plasma, urine, dried fecal extracts, saliva, milk, or cell culture medium.左炔诺孕酮（LNG）ELISA对血清，血浆，尿液，粪便干提取物，唾液，牛奶或细胞培养基中的LNG进行定量。Principle of the method 方法原理The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum, plasma, saliva, urine, dried fecal samples, milk, and tissue culture media samples. This kit measures total LNG in extracted serum, plasma, milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.左炔诺孕酮（LNG）竞争性ELISA试剂盒用于定量测量血清，血浆，唾液，尿液，粪便干粉，牛奶和组织培养基中的左炔诺孕酮。该试剂盒可测量提取的血清，血浆，牛奶和粪便样品中的总液化天然气。提供左炔诺孕酮储备溶液以生成测定的标准曲线，所有样品均应从标准曲线中读出。将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获兔抗体。将左炔诺孕酮-过氧化物酶结合物添加到孔中的标准品和样品中。通过向每个孔中添加左炔诺孕酮的兔多克隆抗体来启动结合反应。温育1小时后，洗涤板并加入底物。底物与结合的左炔诺孕酮-过氧化物酶结合物反应。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.左炔诺孕酮（4-Estren-17alpha-乙炔基-18-homo-17beta-ol-3-one（LNG）是合成的类固醇类似物。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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