Axl Mouse ELISA Kit/小鼠Axl ELISA试剂盒
货号：EMAXL,EMAXLX5,EMAXLX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 1 pg/mL |
| 检测范围 | 1.0-250 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | AXL |
| Protein aliases | Adhesion-related kinase, Tyrosine-protein kinase receptor UFO, ufo oncogene homolog |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AI323647, Ark, Axl, Tyro7, Ufo |
| Gene ID | (Mouse) 26362 |
| Gene symbol | Axl |
| UniProt ID | (Mouse) Q00993 |
| The Mouse Axl ELISA quantitates Ms Axl in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Axl.小鼠Axl ELISA定量检测小鼠血清，血浆或细胞培养基中的Ms Axl。 该检测试剂盒将专门识别天然和重组Ms Axl。Principle of the method 方法原理The Mouse Axl solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠Axl固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息AXL (also known as tyrosine-protein kinase receptor UFO) is a cell surface receptor. The AXL receptor transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6 (GAS6). It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. AXL is an essential epithelial-to-mesenchymal transition-induced regulator of breast cancer metastasis.AXL（也称为酪氨酸蛋白激酶受体UFO）是细胞表面受体。 AXL受体通过结合诸如维生素K依赖蛋白的生长抑制特异性基因6（GAS6）等生长因子，将信号从细胞外基质传导到细胞质中。 它参与细胞增殖的刺激。 该受体还可以通过同源结合介导细胞聚集。 AXL是乳腺癌上皮细胞向间充质转化诱导的重要调节剂。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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