MMP8 Rat ELISA Kit/大鼠MMP8 ELISA试剂盒
货号：ERMMP8,ERMMP8X5
规格：96 tests,5X96 tests
价格：7238,29826
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 50 pg/mL |
| 检测范围 | 0.05-40 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 1 μL 血浆 1 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | MMP8 |
| Protein aliases | Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase, neutrophil collagenease |
物种（Tested） | 大鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Mmp8 |
| Gene ID | (Rat) 63849 |
| Gene symbol | Mmp8 |
| UniProt ID | (Rat) O88766 |
| The Rat Matrix MetalloProteinase 8 (Rt MMP8) ELISA quantitates Rt MMP8 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt MMP8.大鼠基质金属蛋白酶8（大鼠MMP8）ELISA可以定量大鼠血清，血浆或细胞培养基中的大鼠MMP8。 该检测试剂盒将专门识别天然和重组大鼠MMP8。Principle of the method 方法原理The Rat MMP8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠MMP8固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Matrix Metalloproteinase 8 (MMP8) is also known as neutrophil collagenase and collagenase 2. MMP8 degrades fibrillar collagens types I, II, III, aggrecan, serpins and alpha 2 macroglobulin. All collagenases cleave fibrillar collagens at one specific site resulting in generation of N-terminal three-quarter and C-terminal one-quarter fragments, which then denature to gelatin at body temperature. The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen. MMP13, in turn degrades type II collagen 6-fold more efficiently than type I and type II collagens and displays almost 50-fold stronger gelatinolytic activity than MMP1 and MMP8. MMP8 is very similar to MMP1, sharing 57 % amino acid identity. Most cell types do not produce MMP8. Until recently, it was thought that MMP8 was produced exclusively by neutrophils, but it has also been detected in other cell types including arthritic chondrocytes and gingival fibroblasts. The human MMP8 gene has the chromosomal location of 11q22.2-22.3.基质金属蛋白酶8（MMP8）也被称为嗜中性粒细胞胶原酶和胶原酶2。MMP8降解I，II，III型聚集蛋白聚糖，丝氨酸蛋白酶抑制剂和alpha 2巨球蛋白。所有胶原酶都在一个特定位点切割原纤维胶原蛋白，从而生成N末端四分之三和C末端四分之一的片段，然后在体温下变性为明胶。胶原酶的底物特异性是可变的：MMP1比I型或II型胶原更有效地降解III型胶原，而MMP8在降解I型胶原方面比III型或II型胶原更有效。与之相比，MMP13降解II型胶原的效率比I型和II型胶原高6倍，并且明胶分解活性比MMP1和MMP8强近50倍。 MMP8与MMP1非常相似，具有57％的氨基酸同一性。大多数细胞类型不产生MMP8。直到最近，人们还认为MMP8仅由嗜中性粒细胞产生，但也已在其他细胞类型中检测到，包括关节炎软骨细胞和牙龈成纤维细胞。人MMP8基因的染色体位置为11q22.2-22.3。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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