Amphiregulin (AREG) Mouse ELISA Kit/小鼠两性调节蛋白(AREG) ELISA试剂盒
货号：EMAREG,EMAREGX5,EMAREGX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 1.1-800 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Amphiregulin |
| Protein aliases | Amphiregulin, AR, Schwannoma-derived growth factor, SDGF |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | AR, Areg, Mcub, Sdgf |
| Gene ID | (Mouse) 11839 |
| Gene symbol | Areg |
| UniProt ID | (Mouse) P31955 |
| The Mouse Amphiregulin (Ms AREG) ELISA quantitates Ms AREG in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms AREG.小鼠两性蛋白（Ms AREG）ELISA定量检测小鼠血清，血浆或细胞培养基中的Ms AREG。 该检测试剂盒将专门识别天然和重组Ms AREG。Principle of the method 方法原理The Mouse AREG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠AREG固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and beta cellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by six evenly spaced cysteine residues that generate three loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.两性调节蛋白（AR）与EGF受体（EGFR）的亲和力低于EGF。 成熟的分泌形式AR是84个氨基酸残基的糖基化多肽生长调节剂，其通过252个氨基酸的跨膜前体的蛋白水解加工而产生。 源自不同基因的七个不同多肽配体能够结合EGFR的胞外域。 这些配体包括EGF，TGFalpha，AR，HB-EGF，cripto-1，上皮调节蛋白和β细胞蛋白。 所有这些生长因子都包含一个特征性的EGF样结构域，该结构域由六个均匀间隔的半胱氨酸残基定义，这些残基通过形成二硫键生成三个环。 通过免疫组织化学将AR蛋白定位于结肠，胃，胰腺，乳房和胎盘的上皮。 据报道，AR在乳腺癌，结肠癌，胃癌和胰腺癌的人类癌症中过表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20591.htm
QQ:1749072012