ShhN (SHH)-Cell Lysates Mouse ELISA Kit/小鼠ShhN (SHH)-细胞裂解液ELISA试剂盒
货号：EMSHHCL,EMSHHCLX5,EMSHHCLX10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29826,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 5 pg/mL |
| 检测范围 | 4.92-1200 pg/mL |
样本类型/体积 | 细胞裂解液 20 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 细胞裂解液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | SHH |
| Protein aliases | hemimelic extra toes, HHG-1, SHH, Shh N-terminal processed signaling domains, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, Sonic hedgehog protein 19 kDa product, Sonic hedgehog protein 27 kDa product, ShhN, ShhNC, ShhNp, short digits, Sonic hedgehog protein, Sonic hedgehog protein N-product |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | 9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M100081, Shh |
| Gene ID | (Mouse) 20423 |
| Gene symbol | Shh |
| UniProt ID | (Mouse) Q62226 |
| The Mouse ShhN (SHH) ELISA quantitates Ms ShhN in mouse cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Ms ShhN.小鼠ShhN（SHH）ELISA定量检测小鼠细胞或组织裂解物中的ShhN。 该检测试剂盒将专门识别天然和重组Ms ShhN。Principle of the method 方法原理The Mouse ShhN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠ShhN固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.仅在胚胎发生过程中表达的声波刺猬（SHH）有助于构图早期胚胎。它已被认为是腹侧神经管，前后肢轴和腹侧骨节的模式中的关键诱导信号。在三种与果蝇的Sonic Hedgehog蛋白显示序列和功能相似性的人类蛋白中，该蛋白最相似。将该蛋白质制成可被自身催化裂解的前体，N端部分可溶并包含信号传导活性，而C端部分参与前体加工。更重要的是，C末端产物将胆固醇部分共价附于N末端产物，从而将N末端产物限制在细胞表面并阻止其在整个发育中的胚胎中自由扩散。该蛋白或其信号传导途径的缺陷是全脑性前脑（HPE）的病因，该疾病中发育中的前脑未能正确分为左右半球。 HPE表现为面部畸形。另外，人们认为该基因或其信号传导途径的突变可能是VACTERL综合征的原因，VACTERL综合征的特征是椎骨缺损，肛门闭锁，气管食管瘘合并食管闭锁，radial骨和肾发育异常，心脏异常和四肢异常。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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