FLT-3L Monkey ELISA Kit/猴FLT-3L ELISA试剂盒
货号：EPFLT3LG,EPFLT3LGX5
规格：96 tests,5X96 tests
价格：7238,29826
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：恒河猴/小鼠/大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 12 pg/mL |
| 检测范围 | 16.38-4000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | FLT3LG |
| Protein aliases | Flt3 ligand, fms-related tyrosine kinase 3 ligand |
物种（Tested） | 恒河猴 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | FLT3L |
| Gene ID | (Rhesus monkey) 719239 |
| Gene symbol | FLT3LG |
| The Monkey Flt-3 Ligarnd ELISA quantitates Rhesus Mk Flt-3L in monkey serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk Flt-3L.猴 Flt-3 Ligarnd ELISA定量分析了猴血清，血浆或细胞培养基中的猴Flt-3L。 该检测试剂盒将专门识别天然和重组猴Flt-3L。Principle of the method 方法原理The Monkey Flt-3L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.猴Flt-3L固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Flt3 (FMS-related tyrosine kinase 3) Ligand is a growth factor that stimulates the growth and differentiation of early hematopoetic progenitor cells. Flt3 Ligand alone exerts weak biological effects, and instead synergizes with other colony-stimulating factors to induce the proliferation and differentiation of cells of the myeloid and lymphoid lineages. Recent studies have suggested that Flt3 Ligand plays an important role in the development and expansion of dendritic cells in vivo, and that it may influence NK cell survival by this mechanism.Flt3 Ligand transcripts are detectable in most cell types and tissues, although the protein has only been found in stromal cells and T cells. It is expressed most often as a transmembrane cell surface protein, although processing can yield a soluble, secreted isoform.Flt3（与FMS相关的酪氨酸激酶3）配体是一种生长因子，可刺激早期造血祖细胞的生长和分化。 单独的Flt3配体生物学作用弱，与其他集落刺激因子协同作用，以诱导髓样和淋巴谱系细胞的增殖和分化。 研究表明，Flt3配体在体内树突状细胞的发育和扩展中起着重要作用，并且通过这种机制影响NK细胞的存活.Flt3配体的转录本可在大多数细胞类型和组织中检测到，尽管该蛋白仅在基质细胞和T细胞中发现。加工可产生可溶的，分泌的同工型，常表达为跨膜细胞表面蛋白。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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