OPG (TNFRSF11B) Mouse ELISA Kit/小鼠OPG (TNFRSF11B) ELISA试剂盒

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OPG (TNFRSF11B) Mouse ELISA Kit/小鼠OPG (TNFRSF11B) ELISA试剂盒

货号:EMTNFRSF11B,EMTNFRSF11BX5,EMTNFRSF11BX10

规格:96 tests,5X96 tests,10X96 tests

价格:7238,29891,52892

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1 pg/mL

检测范围1.65-1200 pg/mL

样本类型/体积

上清液 100 μL

血清 5 μL

血浆 5 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 预包被96孔板

- 标准品

- 分析稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameTNFRSF11B
Protein aliasesOsteoclastogenesis inhibitory factor, Osteoprotegerin, Tumor necrosis factor receptor superfamily member 11B

物种(Tested)

小鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesOCIF, Opg, Tnfrsf11b, TR1
Gene ID(Mouse) 18383
Gene symbolTnfrsf11b
UniProt ID

(Mouse) O08712

The Mouse Osteoprotegerin (OPG/TNFRSF11B) ELISA quantitates Ms OPG in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms OPG.小鼠骨保护素(OPG / TNFRSF11B)ELISA定量检测小鼠血清,血浆或细胞培养基中的OPG。 该检测试剂盒将专门识别天然和重组Ms OPG。Principle of the method 方法原理The Mouse OPG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠OPG固相夹心ELISA(酶联免疫吸附测定)是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.充当TNFSF11 / RANKL的诱饵受体,从而中和其在破骨细胞形成中的功能。 在体外抑制破骨细胞的活化并促进破骨细胞凋亡。 骨稳态似乎取决于TNFSF11和TNFRSF11B之间的局部比率。 可能还可以预防动脉钙化。 可能充当TNFSF10 / TRAIL的诱饵受体,并防止凋亡。 TNFSF10 / TRAIL结合可阻止破骨细胞生成。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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