Activin A (INHBA) Rat ELISA Kit/大鼠激活素A (INHBA) ELISA试剂盒
货号：ERINHBA,ERINHBAX5
规格：96 tests,5X96 tests
价格：7238,29826
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 2 pg/mL |
| 检测范围 | 1.64-400 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 33 μL 血浆 33 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Activin A |
| Protein aliases | activin A, Activin beta-A chain, Inhibin beta A chain |
物种（Tested） | 大鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Inhba |
| Gene ID | (Rat) 29200 |
| Gene symbol | Inhba |
| UniProt ID | (Rat) P18331 |
| The Rat Activin A (INHBA) ELISA quantitates Rt Activin A in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Activin A.大鼠激活素A（INHBA）ELISA定量了大鼠血清，血浆或细胞培养基中的大鼠激活素A。 该检测试剂盒将专门识别天然和重组大鼠激活素A。Principle of the method 方法原理The Rat Activin A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠激活素A固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive.激活素是二聚体生长和分化因子，属于结构相关信号蛋白的转化生长因子-β（TGF-beta）超家族。 激活素通过受体丝氨酸激酶的异聚复合物发出信号，所述受体丝氨酸激酶包括至少两种I型（I和IB）和两种II型（II和IIB）受体。 这些受体都是跨膜蛋白，由具有富含半胱氨酸区的配体结合细胞外结构域，跨膜结构域和具有预测的丝氨酸/苏氨酸特异性的胞质结构域组成。 I型受体对于信号转导至关重要。 II型和II型受体对于结合配体和I型受体的表达是必需的。 I型和II型受体在配体结合后形成稳定的复合物，导致I型受体被II型受体磷酸化。 激活素A突变与进行性骨化性增生有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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