BHQ-2 T Linker Arm, CPG; 500 Å
货号：BG5-5042TN-1,BG5-5042TN-100
规格：1 g,100mg
价格：35175,4221
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ®-2 T-Linker 500Å CPG is used to add the non-fluorescent quencher (BHQ-2) to the 3' end of an oligonucleotide. BHQ-2 quencher has maximal absorption in the 560 to 670 nm range, which provides excellent quenching of fluorophores emitting in this range, such as TAMRA, Cy3, and CAL Fluor® Red 610. The BHQ-2 T-Linker Arm CPG has a 3' phosphate followed by a glycolate linkage to the CPG support, which allows for rapid cleavage of the oligo from the support and is labile enough for base-sensitive oligonucleotides. The 500 Å CPG is useful for the synthesis of oligos up to 50-mers in length, especially when larger amounts of product are desired, as it allows for greater nucleoside loading amounts than do supports with larger pore sizes. |
| 特性： |
| - Appearance:dark purple powder- Absorption Maximum (Lambda Max):592- Extinction Coefficient at Lambda max:44000- Extinction Coefficient at 260 nm:26100 |
| 用途： |
Cleavage conditions: When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1)for 5 min at 25 °C; or
t-butylamine/H2O(1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O(1:3) for 6 hours at 60 °C. The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:1100.96 |
| 数据： |
| |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20636.htm
QQ:1749072012