MIP-3 beta (CCL19) Mouse ELISA Kit/小鼠MIP-3 β (CCL19) ELISA试剂盒
货号：EMCCL19,EMCCL19X5,EMCCL19X10
规格：96 tests,5X96 tests,10X96 tests
价格：7238,29891,52892
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 5 pg/mL |
| 检测范围 | 4.1-1000 pg/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | <12% |
| 批内变异系数（CV） | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | CCL19 |
| Protein aliases | C-C motif chemokine 19, chemokine CCL19, EBI-1 ligand chemokine, EBI1 ligand chemokine, EBI1-ligand chemokine, EBV-induced molecule 1 ligand chemokine, Epstein-Barr virus-induced molecule 1 ligand chemokine, small inducible cytokine A19, Small-inducible cytokine A19 |
物种（Tested） | 小鼠 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | Ccl19, CKb11, ELC, exodus-3, MIP3B, Scya19 |
| Gene ID | (Mouse) 24047 |
| Gene symbol | Ccl19 |
| UniProt ID | (Mouse) O70460 |
| The Mouse Macrophage Inflammatory Protein 3 beta (CCL19) (Ms MIP-3β) ELISA quantitates Ms MIP-3β in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MIP-3β.小鼠巨噬细胞炎性蛋白3 beta（CCL19）（MsMIP-3β）ELISA定量检测小鼠血清，血浆或细胞培养基中的MsMIP-3β。 该检测试剂盒将专门识别天然和重组MsMIP-3β。Principle of the method 方法原理The Mouse MIP-3β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠MIP-3β固相夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.CCL19，也称为MIP-3 beta（巨噬细胞炎性蛋白3 beta），是趋化因子CC亚家族的成员。 它与CCL21最紧密相关，与CCL21具有32％的氨基酸序列同源性。 CCL19和CCL21主要由在淋巴结的T细胞富集区中的基质细胞表达。 它们是天然T细胞和活化的树突状细胞向次级淋巴器官内稳态转运的关键介体。 CCL19和CCL21在T细胞启动和激活以及淋巴细胞募集到发炎组织中也起作用。 两种蛋白均通过G蛋白偶联受体CCR7发出信号，该受体在T细胞和成熟树突状细胞上表达。 尽管CCL19和CCL21都对CCR7表现出相同的亲和力，但只有CCL19的结合才能导致受体的脱敏和内在化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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