Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit/硫酸脱氢表雄酮（DHEA-S）竞争性ELISA试剂盒

货号：EIADHEA,EIADHEAX10

规格：96 tests,10X96 tests

价格：7238,52892

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	90.9 pg/mL
检测范围	96-60,000 pg/mL
样本类型/体积	粪便提取物 50 µL 血浆 1 µL 唾液 25 µL 血清 1 µL 上清液 50 µL 尿液 25 µL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	8.4%
批内变异系数（CV）	7%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 预包被96孔板 - 标准品 - 测定稀释液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	DHEA-S
Protein aliases	Dehydroepiandrosterone sulfate, DHEA sulfate, DHEAS
物种（Tested）	Chemical
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Dehydroepiandrosterone Sulfate (DHEA-S) ELISA quantitates DHEA-Se in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.硫酸脱氢表雄酮（DHEA-S）ELISA定量测定血清，血浆，尿液，唾液，干燥的粪便提取物或细胞培养基中的DHEA-Se。Principle of the method 方法原理The Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Dehydroepiandrosterone sulfate (DHEA-S) in serum, plasma, urine, and saliva samples, and in fecal extracts. The kit will also quantitatively measure DHEA-S present in tissue culture media samples. A DHEA-S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A DHEA-S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to DHEA-S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound DHEA-S-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.硫酸脱氢表雄酮（DHEA-S）竞争性ELISA试剂盒使用专门生成的抗体来测量血清，血浆，尿液和唾液样品以及粪便提取物中的硫酸脱氢表雄酮（DHEA-S）。该试剂盒还将定量测量组织培养基样品中存在的DHEA-S。提供DHEA-S标准品以生成测定的标准曲线，并且应从标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗体的透明微量滴定板中，以捕获羊抗体。将DHEA-S-过氧化物酶偶联物添加到孔中的标准品和样品中。通过向每个孔中添加针对DHEA-S的多克隆抗体来启动结合反应。温育2小时后，洗涤板并加入底物。底物与结合的DHEA-S-过氧化物酶偶联物反应。短暂孵育后，终止反应，并在能够在450 nm处测量的微量滴定板读数器中检测所产生颜色的强度。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex and a precursor in testosterone and estrogen biosynthesis. DHEA-S is identical across all species and so this kit should measure DHEA-S from all sources. It is produced by the addition of a sulfate group to dehydroepiandrosterone (DHEA), catalyzed by the sulfotransferase enzymes, SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.硫酸脱氢表雄酮（DHEA-S）是肾上腺皮质分泌的主要C19类固醇，是睾丸激素和雌激素生物合成的前体。 DHEA-S在所有物种中都是相同的，因此该试剂盒应从所有来源测量DHEA-S。 它是通过在磺基转移酶SULT1A1和SULT1E1催化下，在脱氢表雄酮（DHEA）上加一个硫酸根而制得的，后者也从雌酮中产生硫酸雌酮。 硫酸脱氢表雄酮还可以通过类固醇硫酸酯酶的作用被逆转为脱氢表雄酮。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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