AKT/ERK Activation Multispecies InstantOne ELISA Kit/多物种AKT /ERK InstantOne ELISA试剂盒-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

AKT/ERK Activation Multispecies InstantOne ELISA Kit/多物种AKT /ERK InstantOne ELISA试剂盒

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AKT/ERK Activation Multispecies InstantOne ELISA Kit/多物种AKT /ERK InstantOne ELISA试剂盒

货号：85-86014-11

规格：96 tests

价格：7990

产品类型：InstantOne ELISA

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

样本类型/体积	细胞裂解液 20μL
操作时间	1 hr 20 min
测试时间	1 hr 10 min
Homogenous (no wash)	No
仪器设备	Colorimetric microplate reader 酶标仪
规格	96 Tests
组份	- 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条
储存温度	2-8℃
Protein name	AKT Pan/Erk1/2
物种（Tested）	人
试剂盒类型	InstantOne ELISA
检测抗体偶联物	Biotin
标记（染料）	HRP
Gene ID	(Human) 10000,(Human) 5595,(Human) 207,(Human) 208,(Human) 5594
Gene symbol	AKT3, MAPK3, AKT1, AKT2, MAPK1
Gene aliases	MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, ST K-2, ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRK M3, p44-ERK1, p44-MAPK, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALP HA, HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, ERK, ERK-2, ERK2, ERT1, M APK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK
UniProt ID	(Human) Q9Y243,(Human) P27361,(Human) P31749,(Human) P31751,(Human) P28482

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster ERK 1/2 and AKT 1/2/3 in cell lysates. Detection of rat ERK 1/2 and AKT 1/2/3 is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA精确测量细胞裂解物中磷酸化的人，小鼠和仓鼠的ERK 1/2和AKT 1/2/3。还期望检测到大鼠ERK 1/2和AKT 1/2/3。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. ERK1 (p44, MAPK3) and ERK2 (p42, MAPK2) are both activated via the MAPK/ERK pathway, which is downstream of various Receptor Tyrosine Kinases (RTKs) or GPCRs. MEK, a Ser/Thr and Tyr kinase, activates ERK by phosphorylation of its TxY motifs, namely Thr202/Tyr204 and Thr185/Tyr187 of ERK1 and ERK2, respectively. Once activated, ERK phosphorylates a variety of proteins that regulate cellular processes such as cell division, proliferation, survival, differentiation, apoptosis, motility, and metabolism.细胞外信号调节激酶（ERK）是经典的丝裂素活化蛋白激酶（MAPK）途径的创始成员和关键组成部分。 ERK1（p44，MAPK3）和ERK2（p42，MAPK2）都通过MAPK / ERK途径激活，该途径位于各种受体酪氨酸激酶（RTK）或GPCR的下游。 MEK是一种Ser / Thr和Tyr激酶，通过其TxY基序（分别为ERK1和ERK2的Thr202 / Tyr204和Thr185 / Tyr187）的磷酸化来激活ERK。一旦激活，ERK就会磷酸化多种调节细胞过程的蛋白质，例如细胞分裂，增殖，存活，分化，凋亡，运动性和代谢。AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.AKT以多种形式存在，是被磷酸肌醇3激酶（PI3K）激活的主要激酶之一。 PI3K的激活导致生成磷脂酰肌醇（3,4,5）-三磷酸（PIP3）。这些脂质第二信使结合至AKT的pleckstrin同源结构域，以促进其易位至质膜，以分别通过PDK1和mTOR TORC2复合物在Thr308和Ser473处的磷酸化激活。这两个位点的磷酸化是AKT Ser / Thr激酶活性完全激活所必需的。 AKT使50多种已知底物磷酸化，包括GSK3，AS160，PRAS40，TSC 1，TCS 2，Raf-1，Bad，FOXO转录因子家族和PFK2。由于AKT在众多生物学过程中已确立了关键作用，因此它一直是肿瘤相关药物开发的常见目标。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。

数据：

AKT/ERK Activation Multispecies InstantOne™ ELISA KitRepresentative data for AKT/ERK Activation Multispecies InstantOne™ ELISA. Left: MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated(+) with 10 ng/mL insulin for 10 min. Cells were analyzed for phosphorylated AKT 1/2/3 by InstantOne™ ELISA. Right: A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor) for 3 hours (-) or treated(+) with 10 ng/mL EGF for 10 min. Cells were analyzed for phosphorylated ERK 1/2 by InstantOne™ ELISA.AKT / ERK激活多物种InstantOne™ELISA的代表性数据。左：将MCF7细胞用10 µMwortmannin洗涤，并用10 ng / mL胰岛素刺激（-）或刺激（+）10分钟。通过InstantOne™ELISA分析细胞的磷酸化AKT 1/2/3。右：A431细胞用10 µM AG1478（EGFR激酶抑制剂）预处理3小时（-）或用10 ng / mL EGF处理（+）10分钟。通过InstantOne™ELISA分析细胞的磷酸化ERK 1/2。

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