p38 MAPK (Total/Phospho) Multispecies InstantOne ELISA Kit/多物种p38 MAPK (Total/Phospho) InstantOne ELISA 试剂盒
货号：85-86023-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | p38 MAPK alpha |
物种（Tested） | 人，小鼠 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 1432,(Mouse) 26416 |
| Gene symbol | MAPK14, Mapk14 |
Gene aliases | CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha |
UniProt ID | (Human) Q8TDX0,(Mouse) P47811 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse p38 MAPK in cell lysates. Detection of rat p38 MAPK is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解物中总磷酸化的人和小鼠p38 MAPK的方法。 预计将检测到大鼠p38 MAPK。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.p38 MAPK级联调节多种细胞对应激，炎症和其他信号的反应。 p38 MAPK在非磷酸化形式中相对失活，并通过Thr-Gly-Tyr基序的双重磷酸化迅速激活。 p38 MAPK有四种同工型，它们的组织表达和对上游激活剂和下游效应子的亲和力不同。 当细胞暴露于肿瘤坏死因子，白介素-1，热休克或其他激活刺激时，MAPK激酶-3的激活通过磷酸化发生。 活化的MAPK激酶3/6使p38 MAPK中的Thr180和Tyr182的每个残基磷酸化。 磷酸化p38 MAPK通过磷酸化激活ATF-2，CHOP-1，MEF-2和其他转录因子。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| p38 MAPK (Total/Phospho) Multispecies InstantOne™ ELISA KitRepresentative data for p38 MAPK (Total/Phospho) Multispecies InstantOne™ ELISA. HEK293 cells untreated (-) or treated with 2 µg/mL anisomycin for 30 min (+). Cells were analyzed for total (left) or phosphorylated (right) p38 MAPK by InstantOne™ ELISA.p38 MAPK（总/磷酸）多物种InstantOne™ELISA的代表性数据。 未处理（-）或用2 µg / mL Anisomycin处理30分钟（+）的HEK293细胞。 通过InstantOne™ELISA分析细胞的总p38 MAPK（左）或磷酸化（右）p38 MAPK。 |
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