AKT (Phospho) [pT308] Multispecies InstantOne ELISA Kit/多物种AKT (Phospho) [pT308] InstantOne ELISA试剂盒
货号：85-86044-11
规格：96 tests
价格：8078
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | AKT1 |
物种（Tested） | 人 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 207 |
| Gene symbol | AKT1 |
Gene aliases | AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA |
UniProt ID | (Human) P31749 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 (Thr308) in cell lysates. Detection of rat AKT 1/2/3 (Thr308) is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA经过专门设计，可精确测量细胞裂解物中磷酸化的人，小鼠和仓鼠AKT 1/2/3（Thr308）。 还期望检测大鼠AKT 1/2/3（Thr308）。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]AKT1基因编码的丝氨酸-苏氨酸蛋白激酶在血清饥饿的原代和永生化成纤维细胞中无催化活性。 AKT1和相关的AKT2被血小板衍生的生长因子激活。 激活是快速且特异的，并且被AKT1的pleckstrin同源结构域中的突变所废除。 结果表明，活化是通过磷脂酰肌醇3-激酶发生的。 在发育中的神经系统中，AKT是生长因子诱导的神经元存活的关键介质。 存活因子可以通过激活丝氨酸/苏氨酸激酶AKT1来以转录独立的方式抑制细胞凋亡，然后使磷酸化并使细胞凋亡机制的成分失活。 该基因的突变与变形杆菌综合征有关。 已经发现该基因有多个选择性剪接的转录变体。 [由RefSeq提供，2011年7月]For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| AKT (Phospho) [pT308] Multispecies InstantOne™ ELISA KitRepresentative data for AKT (Phospho) [pT308] Multispecies InstantOne™ ELISA. HEK293 cells were either left untreated (+) or treated with with the PI3K inhibitor Wortmannin (2uM) (-). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 56 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.AKT（磷酸）[pT308] Multispecies InstantOne™ELISA的代表性数据。 HEK293细胞未处理（+）或用PI3K抑制剂Wortmannin（2uM）（-）处理。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的在56 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
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