c-Jun (Phospho) [pS73] Multispecies InstantOne ELISA Kit/多物种c-Jun (Phospho) [pS73] InstantOne ELISA试剂盒
货号：85-86162-11
规格：96 tests
价格：0
产品类型：InstantOne ELISA
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
样本类型/体积 | 细胞裂解液 20μL |
操作时间 | 1 hr 20 min |
测试时间 | 1 hr 10 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric microplate reader 酶标仪 |
规格 | 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条 |
储存温度 | 2-8℃ |
| Protein name | c-Jun |
物种（Tested） | 人，小鼠 |
试剂盒类型 | InstantOne ELISA |
检测抗体偶联物 | Biotin |
标记（染料） | HRP |
| Gene ID | (Human) 3725,(Mouse) 16476 |
| Gene symbol | JUN, Jun |
Gene aliases | AP-1, AP1, c-Jun, AP-1, Junc, c-jun |
UniProt ID | (Human) P05412,(Mouse) P05627 |
| The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human and mouse c-Jun (Ser73) in cell lysates. Detection of rat c-Jun is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解液中磷酸化的人和小鼠c-Jun（Ser73）的。 还期望检测大鼠c-Jun。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.Fos和Jun二聚化形成活化蛋白1（AP-1），该转录因子与多种细胞和病毒基因（包括人类胶原酶，金属硫蛋白）的12-O-十四烷酰佛波醇13-乙酸盐（TPA）反应元件（TRE）结合 IIa，溶血素，白介素2，SV40和多瘤。 Fos和Jun含有“亮氨酸拉链”基序，可以进行二聚化，并具有生物学活性所需的相邻基本结构域。 Fos的功能活性形式与Jun家族成员处于异二聚体状态。 尽管Jun家族成员可以形成功能性同二聚体，但研究表明Fos家族成员不会自我结合，因此不会自己结合DNA。 各种二聚体在激活依赖于AP-1的基因方面的能力各不相同。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。 |
| 数据： |
| c-Jun (Phospho) [pS73] Multispecies InstantOne™ ELISA KitRepresentative data for c-Jun (Phospho) [pS73] Multispecies InstantOne™ ELISA. NIH-3T3 cells were either untreated (-) or treated with anisomycin (500 ng/mL for 30 min) (+). Cell lysates were prepared as described in the InstantOne ELISA User Manual and were analyzed by immunoblotting (left) and phospho-c-Jun (Ser73) InstantOne™ ELISA (right). The intensity of the band at 36 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.c-Jun（磷酸）[pS73] Multispecies InstantOne™ELISA的代表性数据。 NIH-3T3细胞未经处理（-）或用茴香霉素（500 ng / mL，持续30分钟）（+）处理。 如InstantOne ELISA用户手册所述制备细胞裂解液，并通过免疫印迹（左）和phospho-c-Jun（Ser73）InstantOne™ELISA（右）进行分析。 通过免疫印迹检测到的在36 kDa处的条带强度与InstantOne™ELISA吸收值相关。 |
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