AKT/ERK/p70 S6K Activation Multispecies InstantOne ELISA Kit/多物种AKT/ERK/p70 S6K InstantOne ELISA试剂盒-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

AKT/ERK/p70 S6K Activation Multispecies InstantOne ELISA Kit/多物种AKT/ERK/p70 S6K InstantOne ELISA试剂盒

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AKT/ERK/p70 S6K Activation Multispecies InstantOne ELISA Kit/多物种AKT/ERK/p70 S6K InstantOne ELISA试剂盒

货号：85-86018-11

规格：96 tests

价格：8397

产品类型：InstantOne ELISA

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

样本类型/体积	细胞裂解液 20μL
操作时间	1 hr 20 min
测试时间	1 hr 10 min
Homogenous (no wash)	No
仪器设备	Colorimetric microplate reader 酶标仪
规格	96 Tests
组份	- 捕获抗体 - 检测抗体 - InstantOne 检测板 - 细胞裂解液 - 增强剂溶液 - 洗涤液（10X） - 底物试剂（TMB） - 终止液 - 阳性对照细胞裂解物（冻干） - 密封条
储存温度	2-8℃
Protein name	AKT Pan/Erk1/2/p70 S6 Kinase
物种（Tested）	人
试剂盒类型	InstantOne ELISA
检测抗体偶联物	Biotin
标记（染料）	HRP
Gene ID	(Human) 10000,(Human) 5595,(Human) 6198,(Human) 207,(Human) 208,(Human) 5594
Gene symbol	AKT3, MAPK3, RPS6KB1, AKT1, AKT2, MAPK1
Gene aliases	MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2, ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, ERK, ERK-2, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK
UniProt ID	(Human) Q9Y243,(Human) P27361,(Human) P23443,(Human) P31749,(Human) P31751,(Human) P28482

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), AKT 1/2/3 (human, mouse, hamster), and p70 S6K (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA经过专门设计，可精确测量细胞裂解物中磷酸化的ERK 1/2（人类，小鼠，仓鼠），AKT 1/2/3（人类，小鼠，仓鼠）和p70 S6K（人类，小鼠）。还期望在大鼠中检测到这些磷酸化分子。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. MEK activates ERK 1/2 by phosphorylation of its TxY motifs in response to a variety of stimuli, including growth factors and cytokines.细胞外信号调节激酶（ERK）是经典的丝裂素活化蛋白激酶（MAPK）途径的创始成员和关键组成部分。 MEK通过对TxY基序的磷酸化来激活ERK 1/2，以响应多种刺激，包括生长因子和细胞因子。AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity.AKT以多种同工型形式存在，是被磷酸肌醇3激酶（PI3K）激活的主要激酶之一。这些脂质第二信使结合至AKT的pleckstrin同源结构域，以促进其易位至质膜，以分别通过PDK1和mTOR TORC2复合物在Thr308和Ser473处的磷酸化激活。这两个位点的磷酸化是AKT Ser / Thr激酶活性完全激活所必需的。p70 S6 Kinase (p70S6K) is a member of the ribosomal S6 kinase family of Ser/Thr kinases. p70 S6K activity is controlled by multiple signaling pathways, including the MAPK, phosphoinositide-3 kinase (PI3K), and mTOR pathways. This regulation results in the phosphorylation of multiple sites of p70 S6K, including Thr389 within the linker domain, which is required for full activation. Activated p70 S6K phosphorylates several residues on the S6 ribosomal protein, thereby leading to increased protein synthesis.p70 S6激酶（p70S6K）是Ser / Thr激酶的核糖体S6激酶家族的成员。 p70 S6K活性受多种信号传导途径控制，包括MAPK，磷酸肌醇3激酶（PI3K）和mTOR途径。该调节导致p70 S6K的多个位点（包括连接域内的Thr389）的磷酸化，这是完全激活所必需的。活化的p70 S6K使S6核糖体蛋白上的多个残基磷酸化，从而导致蛋白质合成增加。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。

数据：

AKT/ERK/p70 S6K Activation Multispecies InstantOne™ ELISA KitRepresentative data for ERK/AKT/p70 S6K Activation InstantOne™ ELISA. Left: A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed for phosphorylated ERK 1/2/3 by InstantOne™ ELISA. Middle and Right: MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed for phosphorylated AKT 1/2/3 (middle) and p70 S6K (right) by InstantOne™ ELISA.ERK / AKT / p70 S6K激活InstantOne™ELISA的代表性数据。左：A431细胞用10 µM AG1478（一种EGFR激酶抑制剂）进行预处理，洗涤并未经刺激（-）或用10 ng / mL EGF刺激10分钟（+）。通过InstantOne™ELISA分析细胞的磷酸化ERK 1/2/3。中右：用10 µM渥曼青霉素预处理MCF7细胞，洗涤后不刺激（-），或用10 ng / mL胰岛素刺激10分钟（+）。通过InstantOne™ELISA分析细胞的磷酸化AKT 1/2/3（中）和p70 S6K（右）。

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